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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zea mays Glucose-6-phosphate isomerase, cytosolic (PHI1), partial CSB-YP343773ZAX
CSB-EP343773ZAX
CSB-BP343773ZAX
CSB-MP343773ZAX
CSB-EP343773ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Histone H2B.4 CSB-YP343774ZAX
CSB-EP343774ZAX
CSB-BP343774ZAX
CSB-MP343774ZAX
CSB-EP343774ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diguetia canities Mu-diguetoxin-Dc1a CSB-YP343775DDZ
CSB-EP343775DDZ
CSB-BP343775DDZ
CSB-MP343775DDZ
CSB-EP343775DDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Triose phosphate/phosphate translocator, chloroplastic (TPT), partial CSB-YP343776ZAX1
CSB-EP343776ZAX1
CSB-BP343776ZAX1
CSB-MP343776ZAX1
CSB-EP343776ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Chloroplast envelope membrane protein (cemA), partial CSB-YP343777GGV1
CSB-EP343777GGV1
CSB-BP343777GGV1
CSB-MP343777GGV1
CSB-EP343777GGV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Beta-fructofuranosidase 1 (IVR1) CSB-YP343778ZAX
CSB-EP343778ZAX
CSB-BP343778ZAX
CSB-MP343778ZAX
CSB-EP343778ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Todarodes pacificus Retinal-binding protein CSB-YP343779TFR
CSB-EP343779TFR
CSB-BP343779TFR
CSB-MP343779TFR
CSB-EP343779TFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S20-1 (RPS20A) CSB-YP343780DOA
CSB-EP343780DOA
CSB-BP343780DOA
CSB-MP343780DOA
CSB-EP343780DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L9-1 (RPL9B) CSB-YP343781DOA
CSB-EP343781DOA
CSB-BP343781DOA
CSB-MP343781DOA
CSB-EP343781DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apomastus schlingeri U1-cyrtautoxin-As1b CSB-YP343782AMR
CSB-EP343782AMR
CSB-BP343782AMR
CSB-MP343782AMR
CSB-EP343782AMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dermatophagoides farinae Mite allergen Der f 6 (DERF6) CSB-YP343783DCO
CSB-BP343783DCO
CSB-MP343783DCO
CSB-EP343783DCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistocerca americana Lazarillo protein CSB-YP343784SDK
CSB-EP343784SDK
CSB-BP343784SDK
CSB-MP343784SDK
CSB-EP343784SDK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Foot-and-mouth disease virus Genome polyprotein CSB-YP343785FCD
CSB-EP343785FCD
CSB-BP343785FCD
CSB-MP343785FCD
CSB-EP343785FCD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Glycine-rich RNA-binding protein GRP2A CSB-YP343786SZO
CSB-EP343786SZO
CSB-BP343786SZO
CSB-MP343786SZO
CSB-EP343786SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ricinus communis Catalase isozyme 2 (CAT2) CSB-YP343787RMM
CSB-EP343787RMM
CSB-BP343787RMM
CSB-MP343787RMM
CSB-EP343787RMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine dehydrogenase [decarboxylating] A, mitochondrial (GDCSPA), partial CSB-YP343788FBQ
CSB-EP343788FBQ
CSB-BP343788FBQ
CSB-MP343788FBQ
CSB-EP343788FBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ADP,ATP carrier protein (AAC), partial CSB-YP343789KBK1
CSB-EP343789KBK1
CSB-BP343789KBK1
CSB-MP343789KBK1
CSB-EP343789KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus 60S ribosomal protein L5, mitochondrial (RPL5) CSB-YP343790BWD
CSB-EP343790BWD
CSB-BP343790BWD
CSB-MP343790BWD
CSB-EP343790BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoplasma acidophilum 50S ribosomal protein L15e (rpl15e) CSB-YP343791TND
CSB-EP343791TND
CSB-BP343791TND
CSB-MP343791TND
CSB-EP343791TND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Syndecan-2-A (sdc2-a), partial CSB-YP343792XBE
CSB-EP343792XBE
CSB-BP343792XBE
CSB-MP343792XBE
CSB-EP343792XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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