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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium sp. Uncharacterized protein y4lN (NGR_a02620), partial CSB-YP345092RKX1
CSB-EP345092RKX1
CSB-BP345092RKX1
CSB-MP345092RKX1
CSB-EP345092RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4mH (NGR_a02510) CSB-YP345093RKX
CSB-EP345093RKX
CSB-BP345093RKX
CSB-MP345093RKX
CSB-EP345093RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized peptidase y4nA (NGR_a02410), partial CSB-YP345094RKX
CSB-EP345094RKX
CSB-BP345094RKX
CSB-MP345094RKX
CSB-EP345094RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized GMC-type oxidoreductase y4nJ (NGR_a02320), partial CSB-YP345095RKX
CSB-EP345095RKX
CSB-BP345095RKX
CSB-MP345095RKX
CSB-EP345095RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4oD (NGR_a02250) CSB-YP345096RKX
CSB-EP345096RKX
CSB-BP345096RKX
CSB-MP345096RKX
CSB-EP345096RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4oM (NGR_a02220) CSB-YP345097RKX
CSB-EP345097RKX
CSB-BP345097RKX
CSB-MP345097RKX
CSB-EP345097RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4oW (NGR_a02130) CSB-YP345098RKX
CSB-EP345098RKX
CSB-BP345098RKX
CSB-MP345098RKX
CSB-EP345098RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4pM (NGR_a01990) CSB-YP345099RKX
CSB-EP345099RKX
CSB-BP345099RKX
CSB-MP345099RKX
CSB-EP345099RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Putative transposase y4qE (NGR_a01930) CSB-YP345100RKX
CSB-EP345100RKX
CSB-BP345100RKX
CSB-MP345100RKX
CSB-EP345100RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Putative integrase/recombinase y4qK (NGR_a01870) CSB-YP345101RKX
CSB-EP345101RKX
CSB-BP345101RKX
CSB-MP345101RKX
CSB-EP345101RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4rH (NGR_a01790) CSB-YP345102RKX
CSB-EP345102RKX
CSB-BP345102RKX
CSB-MP345102RKX
CSB-EP345102RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized HTH-type transcriptional regulator y4tD (NGR_a01550) CSB-YP345103RKX
CSB-EP345103RKX
CSB-BP345103RKX
CSB-MP345103RKX
CSB-EP345103RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized cyclodeaminase y4tK (NGR_a01480) CSB-YP345104RKX
CSB-EP345104RKX
CSB-BP345104RKX
CSB-MP345104RKX
CSB-EP345104RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4vR (NGR_a01060) CSB-YP345105RKX
CSB-EP345105RKX
CSB-BP345105RKX
CSB-MP345105RKX
CSB-EP345105RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4wF (NGR_a00990) CSB-YP345106RKX
CSB-EP345106RKX
CSB-BP345106RKX
CSB-MP345106RKX
CSB-EP345106RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP345107RKX
CSB-EP345107RKX
CSB-BP345107RKX
CSB-MP345107RKX
CSB-EP345107RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. UPF0460 protein y4xD (NGR_a00860) CSB-YP345108RKX
CSB-EP345108RKX
CSB-BP345108RKX
CSB-MP345108RKX
CSB-EP345108RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4xJ (NGR_a00790) CSB-YP345109RKX
CSB-EP345109RKX
CSB-BP345109RKX
CSB-MP345109RKX
CSB-EP345109RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nodulation outer protein X (nopX), partial CSB-YP345110RKX
CSB-EP345110RKX
CSB-BP345110RKX
CSB-MP345110RKX
CSB-EP345110RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nodulation protein nolV (nolV) CSB-YP345111RKX
CSB-EP345111RKX
CSB-BP345111RKX
CSB-MP345111RKX
CSB-EP345111RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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