Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kluyveromyces lactis Guanine nucleotide-binding protein alpha-2 subunit (GPA2), partial CSB-YP346513KBK
CSB-EP346513KBK
CSB-BP346513KBK
CSB-MP346513KBK
CSB-EP346513KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribonuclease J (Cgl1970, cg2160), partial CSB-YP346514DWY
CSB-EP346514DWY
CSB-BP346514DWY
CSB-MP346514DWY
CSB-EP346514DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class gamma-7 (srg-7), partial CSB-YP346515CXY1
CSB-EP346515CXY1
CSB-BP346515CXY1
CSB-MP346515CXY1
CSB-EP346515CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ML1176 (ML1176), partial CSB-YP346516MVN1
CSB-EP346516MVN1
CSB-BP346516MVN1
CSB-MP346516MVN1
CSB-EP346516MVN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ammonium transporter 1 (amt-1), partial CSB-YP346517CXY
CSB-EP346517CXY
CSB-BP346517CXY
CSB-MP346517CXY
CSB-EP346517CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Peptide methionine sulfoxide reductase (E4) CSB-YP346518SZY
CSB-EP346518SZY
CSB-BP346518SZY
CSB-MP346518SZY
CSB-EP346518SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP346520BRJ
CSB-EP346520BRJ
CSB-BP346520BRJ
CSB-MP346520BRJ
CSB-EP346520BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Hemolysin-3 homolog (yplQ), partial CSB-YP346521BRJ1
CSB-EP346521BRJ1
CSB-BP346521BRJ1
CSB-MP346521BRJ1
CSB-EP346521BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative competence-damage inducible protein (cinA) CSB-YP346522FMW
CSB-EP346522FMW
CSB-BP346522FMW
CSB-MP346522FMW
CSB-EP346522FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Pheromone-binding protein-related protein 2 (Pbprp2) CSB-YP346523DLU
CSB-EP346523DLU
CSB-BP346523DLU
CSB-MP346523DLU
CSB-EP346523DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase MPS1 (MPS1), partial CSB-YP346524SVG
CSB-EP346524SVG
CSB-BP346524SVG
CSB-MP346524SVG
CSB-EP346524SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP346525FPY
CSB-EP346525FPY
CSB-BP346525FPY
CSB-MP346525FPY
CSB-EP346525FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fructose-bisphosphate aldolase 1 (aldo-1) CSB-YP346526CXY
CSB-EP346526CXY
CSB-BP346526CXY
CSB-MP346526CXY
CSB-EP346526CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase, cytosolic 1 (PGIC1), partial CSB-YP346527CLB
CSB-EP346527CLB
CSB-BP346527CLB
CSB-MP346527CLB
CSB-EP346527CLB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase, cytosolic 1 (PGIC1), partial CSB-YP346528CLH
CSB-EP346528CLH
CSB-BP346528CLH
CSB-MP346528CLH
CSB-EP346528CLH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera mexicana Glucose-6-phosphate isomerase, cytosolic (PGIC), partial CSB-YP346529OAT
CSB-EP346529OAT
CSB-BP346529OAT
CSB-MP346529OAT
CSB-EP346529OAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus delbrueckii subsp. bulgaricus Asparagine--tRNA ligase (asnS1) CSB-YP346530LAQ
CSB-EP346530LAQ
CSB-BP346530LAQ
CSB-MP346530LAQ
CSB-EP346530LAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Regulatory protein rhlR (rhlR) CSB-YP346531EZX
CSB-BP346531EZX
CSB-MP346531EZX
CSB-EP346531EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Autoinducer 2 sensor kinase/phosphatase luxQ (luxQ), partial CSB-YP346532VFD
CSB-EP346532VFD
CSB-BP346532VFD
CSB-MP346532VFD
CSB-EP346532VFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phage-like element PBSX protein xkdE (xkdE) CSB-YP346533BRJ
CSB-EP346533BRJ
CSB-BP346533BRJ
CSB-MP346533BRJ
CSB-EP346533BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>