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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanothermobacter marburgensis F420-dependent methylenetetrahydromethanopterin dehydrogenase (mtd) CSB-YP345457MSQ
CSB-EP345457MSQ
CSB-BP345457MSQ
CSB-MP345457MSQ
CSB-EP345457MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Adipokinetic prohormone type 1 CSB-YP345458LHY
CSB-EP345458LHY
CSB-BP345458LHY
CSB-MP345458LHY
CSB-EP345458LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartic protease pep1 (pep1) CSB-YP345459DOZ
CSB-EP345459DOZ
CSB-BP345459DOZ
CSB-MP345459DOZ
CSB-EP345459DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus awamori Endo-1,4-beta-xylanase A (xynA) CSB-YP345460APL
CSB-EP345460APL
CSB-BP345460APL
CSB-MP345460APL
CSB-EP345460APL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Endo-1,4-beta-xylanase 22 (XYN22) CSB-YP345461MPM
CSB-EP345461MPM
CSB-BP345461MPM
CSB-MP345461MPM
CSB-EP345461MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0747 protein yllA (yllA), partial CSB-YP345462BRJ
CSB-EP345462BRJ
CSB-BP345462BRJ
CSB-MP345462BRJ
CSB-EP345462BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4aO (NGR_a00330) CSB-YP345463RKX
CSB-EP345463RKX
CSB-BP345463RKX
CSB-MP345463RKX
CSB-EP345463RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Putative transposase y4bF (NGR_a00240) CSB-YP345464RKX
CSB-EP345464RKX
CSB-BP345464RKX
CSB-MP345464RKX
CSB-EP345464RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4cB (NGR_a00120), partial CSB-YP345465RKX1
CSB-EP345465RKX1
CSB-BP345465RKX1
CSB-MP345465RKX1
CSB-EP345465RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable conjugal transfer protein trbD (trbD), partial CSB-YP345466RKX1
CSB-EP345466RKX1
CSB-BP345466RKX1
CSB-MP345466RKX1
CSB-EP345466RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable conjugal transfer protein trbH (trbH) CSB-YP345467RKX
CSB-EP345467RKX
CSB-BP345467RKX
CSB-MP345467RKX
CSB-EP345467RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized HTH-type transcriptional regulator y4dJ (NGR_a04070) CSB-YP345468RKX
CSB-EP345468RKX
CSB-BP345468RKX
CSB-MP345468RKX
CSB-EP345468RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable conjugal transfer protein traA (traA), partial CSB-YP345469RKX
CSB-EP345469RKX
CSB-BP345469RKX
CSB-MP345469RKX
CSB-EP345469RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4dX (NGR_a03930) CSB-YP345470RKX
CSB-EP345470RKX
CSB-BP345470RKX
CSB-MP345470RKX
CSB-EP345470RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4eI (NGR_a03850), partial CSB-YP345471RKX1
CSB-EP345471RKX1
CSB-BP345471RKX1
CSB-MP345471RKX1
CSB-EP345471RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4fE (NGR_a03760) CSB-YP345472RKX
CSB-EP345472RKX
CSB-BP345472RKX
CSB-MP345472RKX
CSB-EP345472RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable ABC transporter permease protein y4fN (NGR_a03680), partial CSB-YP345473RKX
CSB-EP345473RKX
CSB-BP345473RKX
CSB-MP345473RKX
CSB-EP345473RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4gE (NGR_a03590) CSB-YP345474RKX
CSB-EP345474RKX
CSB-BP345474RKX
CSB-MP345474RKX
CSB-EP345474RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Putative ionic transporter y4hA (NGR_a03490), partial CSB-YP345475RKX
CSB-EP345475RKX
CSB-BP345475RKX
CSB-MP345475RKX
CSB-EP345475RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nodulation protein J (nodJ), partial CSB-YP345476RKX1
CSB-EP345476RKX1
CSB-BP345476RKX1
CSB-MP345476RKX1
CSB-EP345476RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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