Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Non-classical export protein 2 homolog 1 (FHN1), partial CSB-YP346068SVG1
CSB-EP346068SVG1
CSB-BP346068SVG1
CSB-MP346068SVG1
CSB-EP346068SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Polyamine transporter 2 (TPO2), partial CSB-YP346069SVG
CSB-EP346069SVG
CSB-BP346069SVG
CSB-MP346069SVG
CSB-EP346069SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR168C (YGR168C), partial CSB-YP346070SVG1
CSB-EP346070SVG1
CSB-BP346070SVG1
CSB-MP346070SVG1
CSB-EP346070SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae N-acetyltransferase SLI1 (SLI1) CSB-YP346071SVG
CSB-EP346071SVG
CSB-BP346071SVG
CSB-MP346071SVG
CSB-EP346071SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UPF0041 protein FMP43 (FMP43), partial CSB-YP346072SVG1
CSB-EP346072SVG1
CSB-BP346072SVG1
CSB-MP346072SVG1
CSB-EP346072SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase BUD32 (BUD32) CSB-YP346073SVG
CSB-EP346073SVG
CSB-BP346073SVG
CSB-MP346073SVG
CSB-EP346073SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR283C (YGR283C) CSB-YP346074SVG
CSB-EP346074SVG
CSB-BP346074SVG
CSB-MP346074SVG
CSB-EP346074SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Alpha-glucosidase MAL12 (MAL12), partial CSB-YP346075SVG
CSB-EP346075SVG
CSB-BP346075SVG
CSB-MP346075SVG
CSB-EP346075SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes aegypti Alpha-amylase I (AMY1), partial CSB-YP346076AXQ
CSB-EP346076AXQ
CSB-BP346076AXQ
CSB-MP346076AXQ
CSB-EP346076AXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP346077BUD
CSB-EP346077BUD
CSB-BP346077BUD
CSB-MP346077BUD
CSB-EP346077BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ammonium transporter MEP3 (MEP3), partial CSB-YP346078SVG
CSB-EP346078SVG
CSB-BP346078SVG
CSB-MP346078SVG
CSB-EP346078SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Uncharacterized protein CG10349 (CG10349) CSB-YP346079DLU
CSB-EP346079DLU
CSB-BP346079DLU
CSB-MP346079DLU
CSB-EP346079DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Insulin gene enhancer protein isl-2b (isl2b) CSB-YP346080DIL
CSB-EP346080DIL
CSB-BP346080DIL
CSB-MP346080DIL
CSB-EP346080DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phalaenopsis sp. Bibenzyl synthase (BIBSY212) CSB-YP346081PGT
CSB-EP346081PGT
CSB-BP346081PGT
CSB-MP346081PGT
CSB-EP346081PGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ML1138 (ML1138), partial CSB-YP346082MVN1
CSB-EP346082MVN1
CSB-BP346082MVN1
CSB-MP346082MVN1
CSB-EP346082MVN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae RNA polymerase I-specific transcription initiation factor RRN9 (RRN9) CSB-YP346083SVG
CSB-EP346083SVG
CSB-BP346083SVG
CSB-MP346083SVG
CSB-EP346083SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Fructose-bisphosphate aldolase CSB-YP346084SXQ
CSB-EP346084SXQ
CSB-BP346084SXQ
CSB-MP346084SXQ
CSB-EP346084SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diphyllobothrium dendriticum Actin-6 (ACT6) CSB-YP346085DEN
CSB-EP346085DEN
CSB-BP346085DEN
CSB-MP346085DEN
CSB-EP346085DEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Actin-1 CSB-YP346086SXQ
CSB-EP346086SXQ
CSB-BP346086SXQ
CSB-MP346086SXQ
CSB-EP346086SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Actin, alpha anomalous CSB-YP346087TKY
CSB-EP346087TKY
CSB-BP346087TKY
CSB-MP346087TKY
CSB-EP346087TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>