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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium sp. Uncharacterized protein y4fL (NGR_a03700), partial CSB-YP346251RKX1
CSB-EP346251RKX1
CSB-BP346251RKX1
CSB-MP346251RKX1
CSB-EP346251RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4gA (NGR_a03630) CSB-YP346252RKX
CSB-EP346252RKX
CSB-BP346252RKX
CSB-MP346252RKX
CSB-EP346252RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Protein fixF (fixF) CSB-YP346253RKX
CSB-EP346253RKX
CSB-BP346253RKX
CSB-MP346253RKX
CSB-EP346253RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. 2-O-methyltransferase NoeI (noeI) CSB-YP346254RKX
CSB-EP346254RKX
CSB-BP346254RKX
CSB-MP346254RKX
CSB-EP346254RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized oxidoreductase y4hM (NGR_a03370) CSB-YP346255RKX
CSB-EP346255RKX
CSB-BP346255RKX
CSB-MP346255RKX
CSB-EP346255RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4iF (NGR_a03270) CSB-YP346256RKX
CSB-EP346256RKX
CSB-BP346256RKX
CSB-MP346256RKX
CSB-EP346256RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4iL (NGR_a03210) CSB-YP346257RKX
CSB-EP346257RKX
CSB-BP346257RKX
CSB-MP346257RKX
CSB-EP346257RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4jC (NGR_a03130) CSB-YP346258RKX
CSB-EP346258RKX
CSB-BP346258RKX
CSB-MP346258RKX
CSB-EP346258RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable VapC ribonuclease Y4jK (NGR_a03040) CSB-YP346259RKX
CSB-EP346259RKX
CSB-BP346259RKX
CSB-MP346259RKX
CSB-EP346259RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4kF (NGR_a02900) CSB-YP346260RKX
CSB-EP346260RKX
CSB-BP346260RKX
CSB-MP346260RKX
CSB-EP346260RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4kK (NGR_a02850) CSB-YP346261RKX
CSB-EP346261RKX
CSB-BP346261RKX
CSB-MP346261RKX
CSB-EP346261RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Putative uncharacterized protein y4kR (NGR_a02780) CSB-YP346262RKX
CSB-EP346262RKX
CSB-BP346262RKX
CSB-MP346262RKX
CSB-EP346262RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4lO (NGR_a02610) CSB-YP346263RKX
CSB-EP346263RKX
CSB-BP346263RKX
CSB-MP346263RKX
CSB-EP346263RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4mC (NGR_a02560) CSB-YP346264RKX
CSB-EP346264RKX
CSB-BP346264RKX
CSB-MP346264RKX
CSB-EP346264RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4mL (NGR_a02470), partial CSB-YP346265RKX
CSB-EP346265RKX
CSB-BP346265RKX
CSB-MP346265RKX
CSB-EP346265RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized HTH-type transcriptional regulator y4mQ (NGR_a02420) CSB-YP346266RKX
CSB-EP346266RKX
CSB-BP346266RKX
CSB-MP346266RKX
CSB-EP346266RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4oB (NGR_a02270) CSB-YP346267RKX
CSB-EP346267RKX
CSB-BP346267RKX
CSB-MP346267RKX
CSB-EP346267RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable ABC transporter permease protein y4oR (NGR_a02180), partial CSB-YP346268RKX1
CSB-EP346268RKX1
CSB-BP346268RKX1
CSB-MP346268RKX1
CSB-EP346268RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4oV (NGR_a02140) CSB-YP346269RKX
CSB-EP346269RKX
CSB-BP346269RKX
CSB-MP346269RKX
CSB-EP346269RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4pG/y4sC (NGR_a02050) CSB-YP346270RKX
CSB-EP346270RKX
CSB-BP346270RKX
CSB-MP346270RKX
CSB-EP346270RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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