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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Lipoprotein yhcN (yhcN) CSB-YP346188BRJ
CSB-EP346188BRJ
CSB-BP346188BRJ
CSB-MP346188BRJ
CSB-EP346188BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cell division control protein 48 homolog A (CDC48A), partial CSB-YP346189DOA
CSB-EP346189DOA
CSB-BP346189DOA
CSB-MP346189DOA
CSB-EP346189DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Trypsin delta/gamma (deltaTry) CSB-YP346191DLK
CSB-EP346191DLK
CSB-BP346191DLK
CSB-MP346191DLK
CSB-EP346191DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cysteine proteinase 4 (cprD) CSB-YP346192DKK
CSB-EP346192DKK
CSB-BP346192DKK
CSB-MP346192DKK
CSB-EP346192DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis Transcriptional regulatory protein DegU (degU) CSB-YP346193BWK
CSB-EP346193BWK
CSB-BP346193BWK
CSB-MP346193BWK
CSB-EP346193BWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E6 (E6) CSB-YP346194HOK
CSB-EP346194HOK
CSB-BP346194HOK
CSB-MP346194HOK
CSB-EP346194HOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Fimbrin (fimA), partial CSB-YP346195DKK
CSB-EP346195DKK
CSB-BP346195DKK
CSB-MP346195DKK
CSB-EP346195DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator glvR (glvR) CSB-YP346196BRJ
CSB-EP346196BRJ
CSB-BP346196BRJ
CSB-MP346196BRJ
CSB-EP346196BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative oxidoreductase CatD (catD), partial CSB-YP346197BRJ1
CSB-EP346197BRJ1
CSB-BP346197BRJ1
CSB-MP346197BRJ1
CSB-EP346197BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine-protein kinase pkn2 (pkn2), partial CSB-YP346198MXA
CSB-EP346198MXA
CSB-BP346198MXA
CSB-MP346198MXA
CSB-EP346198MXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Serine/threonine-protein kinase pkaA (pkaA), partial CSB-YP346199FOB
CSB-EP346199FOB
CSB-BP346199FOB
CSB-MP346199FOB
CSB-EP346199FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP-binding nuclear protein Ran1B (RAN1B) CSB-YP346200LQQ
CSB-EP346200LQQ
CSB-BP346200LQQ
CSB-MP346200LQQ
CSB-EP346200LQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaver somniferum Tyrosine/DOPA decarboxylase 5 (TYDC5), partial CSB-YP346201ERH
CSB-EP346201ERH
CSB-BP346201ERH
CSB-MP346201ERH
CSB-EP346201ERH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 26S protease regulatory subunit 6A homolog (TBP1) CSB-YP346202SZY
CSB-EP346202SZY
CSB-BP346202SZY
CSB-MP346202SZY
CSB-EP346202SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 9 (VPS9) CSB-YP346203SVG
CSB-EP346203SVG
CSB-BP346203SVG
CSB-MP346203SVG
CSB-EP346203SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Protein moaF (moaF) CSB-YP346204EKN
CSB-EP346204EKN
CSB-BP346204EKN
CSB-MP346204EKN
CSB-EP346204EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transitional endoplasmic reticulum ATPase homolog 2 (cdc-48.2), partial CSB-YP346206CXY
CSB-EP346206CXY
CSB-BP346206CXY
CSB-MP346206CXY
CSB-EP346206CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apple chlorotic leaf spot virus RNA-directed RNA polymerase, partial CSB-YP346208AMS
CSB-EP346208AMS
CSB-BP346208AMS
CSB-MP346208AMS
CSB-EP346208AMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable gamma-glutamyl phosphate reductase (proA) CSB-YP346209SSQ
CSB-EP346209SSQ
CSB-BP346209SSQ
CSB-MP346209SSQ
CSB-EP346209SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Hydroxyneurosporene methyltransferase (crtF) CSB-YP346210RLF
CSB-EP346210RLF
CSB-BP346210RLF
CSB-MP346210RLF
CSB-EP346210RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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