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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human herpesvirus 7 Tripartite terminase subunit UL15 homolog (U66/U60), partial CSB-YP346761HKB
CSB-EP346761HKB
CSB-BP346761HKB
CSB-MP346761HKB
CSB-EP346761HKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A DNA helicase/primase complex protein (U43), partial CSB-YP346762HJZ
CSB-EP346762HJZ
CSB-BP346762HJZ
CSB-MP346762HJZ
CSB-EP346762HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ubiquitin-conjugating enzyme E2 21 (ubc-21) CSB-YP346763CXY
CSB-EP346763CXY
CSB-BP346763CXY
CSB-MP346763CXY
CSB-EP346763CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Ubiquitin-conjugating enzyme E2 2 (mus-8) CSB-YP346764NHA
CSB-EP346764NHA
CSB-BP346764NHA
CSB-MP346764NHA
CSB-EP346764NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murid herpesvirus 1 Envelope glycoprotein L (GL) CSB-YP346765MJT
CSB-EP346765MJT
CSB-BP346765MJT
CSB-MP346765MJT
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 7 Protein U25 (U25) CSB-YP346766HKB
CSB-EP346766HKB
CSB-BP346766HKB
CSB-MP346766HKB
CSB-EP346766HKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6B Protein U84 (U84) CSB-YP346767HKA
CSB-EP346767HKA
CSB-BP346767HKA
CSB-MP346767HKA
CSB-EP346767HKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6B Envelope glycoprotein H (gH), partial CSB-YP346768HKA1
CSB-BP346768HKA1
CSB-MP346768HKA1
CSB-EP346768HKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable prefoldin subunit 6 (pfd-6) CSB-YP346769CXY
CSB-EP346769CXY
CSB-BP346769CXY
CSB-MP346769CXY
CSB-EP346769CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 N5-carboxyaminoimidazole ribonucleotide mutase (purE) CSB-YP346770BMO
CSB-EP346770BMO
CSB-BP346770BMO
CSB-MP346770BMO
CSB-EP346770BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tortula ruralis Probable 1-Cys peroxiredoxin CSB-YP346771TGX
CSB-EP346771TGX
CSB-BP346771TGX
CSB-MP346771TGX
CSB-EP346771TGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Isoflavone reductase homolog A622 CSB-YP346772NHE
CSB-EP346772NHE
CSB-BP346772NHE
CSB-MP346772NHE
CSB-EP346772NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Vascular endothelial growth factor receptor 2 (KDR), partial CSB-YP346773DXJ
CSB-EP346773DXJ
CSB-BP346773DXJ
CSB-MP346773DXJ
CSB-EP346773DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phase 2 flagellin (fljB) CSB-YP346774SBC
CSB-EP346774SBC
CSB-BP346774SBC
CSB-MP346774SBC
CSB-EP346774SBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Putative amino-acid ABC transporter-binding protein patH (patH) CSB-YP346775VFD
CSB-EP346775VFD
CSB-BP346775VFD
CSB-MP346775VFD
CSB-EP346775VFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative electron transport protein yccM (yccM), partial CSB-YP346776ENV1
CSB-EP346776ENV1
CSB-BP346776ENV1
CSB-MP346776ENV1
CSB-EP346776ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP346777SSQ
CSB-EP346777SSQ
CSB-BP346777SSQ
CSB-MP346777SSQ
CSB-EP346777SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Heat shock protein hslJ (hslJ) CSB-YP346778ENV
CSB-EP346778ENV
CSB-BP346778ENV
CSB-MP346778ENV
CSB-EP346778ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional activator AmpR (ampR) CSB-YP346779CKT
CSB-EP346779CKT
CSB-BP346779CKT
CSB-MP346779CKT
CSB-EP346779CKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP346780TEB
CSB-EP346780TEB
CSB-BP346780TEB
CSB-MP346780TEB
CSB-EP346780TEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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