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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGL218W (YGL218W) CSB-YP347167SVG
CSB-EP347167SVG
CSB-BP347167SVG
CSB-MP347167SVG
CSB-EP347167SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein YIP4 (YIP4), partial CSB-YP347168SVG1
CSB-EP347168SVG1
CSB-BP347168SVG1
CSB-MP347168SVG1
CSB-EP347168SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cystathionine beta-lyase (STR3) CSB-YP347169SVG
CSB-BP347169SVG
CSB-MP347169SVG
CSB-EP347169SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGL165C (YGL165C), partial CSB-YP347170SVG1
CSB-EP347170SVG1
CSB-BP347170SVG1
CSB-MP347170SVG1
CSB-EP347170SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGL152C (YGL152C), partial CSB-YP347171SVG1
CSB-EP347171SVG1
CSB-BP347171SVG1
CSB-MP347171SVG1
CSB-EP347171SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Methylenetetrahydrofolate reductase 2 (MET13), partial CSB-YP347172SVG
CSB-EP347172SVG
CSB-BP347172SVG
CSB-MP347172SVG
CSB-EP347172SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome biogenesis protein NSA1 (NSA1) CSB-YP347173SVG
CSB-EP347173SVG
CSB-BP347173SVG
CSB-MP347173SVG
CSB-EP347173SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGL102C (YGL102C) CSB-YP347174SVG
CSB-EP347174SVG
CSB-BP347174SVG
CSB-MP347174SVG
CSB-EP347174SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Homeobox protein TOS8 (TOS8) CSB-YP347175SVG
CSB-EP347175SVG
CSB-BP347175SVG
CSB-MP347175SVG
CSB-EP347175SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGL069C (YGL069C) CSB-YP347176SVG
CSB-EP347176SVG
CSB-BP347176SVG
CSB-MP347176SVG
CSB-EP347176SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae NADH pyrophosphatase (NPY1) CSB-YP347177SVG
CSB-EP347177SVG
CSB-BP347177SVG
CSB-MP347177SVG
CSB-EP347177SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae rRNA-processing protein CGR1 (CGR1) CSB-YP347178SVG
CSB-EP347178SVG
CSB-BP347178SVG
CSB-MP347178SVG
CSB-EP347178SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR011W (YGR011W) CSB-YP347179SVG
CSB-EP347179SVG
CSB-BP347179SVG
CSB-MP347179SVG
CSB-EP347179SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Probable transcriptional regulatory protein HAH1 (HAH1) CSB-YP347180SVG
CSB-EP347180SVG
CSB-BP347180SVG
CSB-MP347180SVG
CSB-EP347180SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA (His) guanylyltransferase CSB-YP347181SVG
CSB-EP347181SVG
CSB-BP347181SVG
CSB-MP347181SVG
CSB-EP347181SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGR039W (YGR039W) CSB-YP347182SVG
CSB-EP347182SVG
CSB-BP347182SVG
CSB-MP347182SVG
CSB-EP347182SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Arrestin-related trafficking adapter 5 (ART5), partial CSB-YP347183SVG
CSB-EP347183SVG
CSB-BP347183SVG
CSB-MP347183SVG
CSB-EP347183SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae CWF19-like protein DRN1 (DRN1), partial CSB-YP347184SVG
CSB-EP347184SVG
CSB-BP347184SVG
CSB-MP347184SVG
CSB-EP347184SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein CAF130 (CAF130), partial CSB-YP347185SVG
CSB-EP347185SVG
CSB-BP347185SVG
CSB-MP347185SVG
CSB-EP347185SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR151C (YGR151C) CSB-YP347186SVG
CSB-EP347186SVG
CSB-BP347186SVG
CSB-MP347186SVG
CSB-EP347186SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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