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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zinc metalloproteinase nas-7 (nas-7) CSB-YP347004CXY
CSB-EP347004CXY
CSB-BP347004CXY
CSB-MP347004CXY
CSB-EP347004CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Leukotoxin-activating lysine-acyltransferase lktC serotype T3 (lktC) CSB-YP347005ESE
CSB-EP347005ESE
CSB-BP347005ESE
CSB-MP347005ESE
CSB-EP347005ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (uracil (54)-C (5))-methyltransferase CSB-YP347006NFZ
CSB-EP347006NFZ
CSB-BP347006NFZ
CSB-MP347006NFZ
CSB-EP347006NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Phytochrome A (PHYA), partial CSB-YP347007PGL
CSB-EP347007PGL
CSB-BP347007PGL
CSB-MP347007PGL
CSB-EP347007PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein dltD (dltD) CSB-YP347008LMW
CSB-EP347008LMW
CSB-BP347008LMW
CSB-MP347008LMW
CSB-EP347008LMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein alpha chain (nifD2), partial CSB-YP347009MSL
CSB-EP347009MSL
CSB-BP347009MSL
CSB-MP347009MSL
CSB-EP347009MSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yxjO (yxjO) CSB-YP347010BRJ
CSB-EP347010BRJ
CSB-BP347010BRJ
CSB-MP347010BRJ
CSB-EP347010BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna aconitifolia Phosphoribosylaminoimidazole carboxylase, chloroplastic (PURKE), partial CSB-YP347011VCU
CSB-EP347011VCU
CSB-BP347011VCU
CSB-MP347011VCU
CSB-EP347011VCU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa O-succinylhomoserine sulfhydrylase (metZ) CSB-YP347012EZX
CSB-EP347012EZX
CSB-BP347012EZX
CSB-MP347012EZX
CSB-EP347012EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glucose-1-phosphate adenylyltransferase large subunit 1, chloroplastic (ADG2) CSB-YP347013DOA
CSB-EP347013DOA
CSB-BP347013DOA
CSB-MP347013DOA
CSB-EP347013DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannan endo-1,4-beta-mannosidase B (MANB), partial CSB-YP347014PKT
CSB-EP347014PKT
CSB-BP347014PKT
CSB-MP347014PKT
CSB-EP347014PKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catalase R (catR), partial CSB-YP347015DOZ
CSB-EP347015DOZ
CSB-BP347015DOZ
CSB-MP347015DOZ
CSB-EP347015DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Gonad-inhibiting hormone CSB-YP347016HWO
CSB-EP347016HWO
CSB-BP347016HWO
CSB-MP347016HWO
CSB-EP347016HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Humicola insolens Endo-1,4-beta-xylanase 1 (XYL1) CSB-YP347017HQN
CSB-EP347017HQN
CSB-BP347017HQN
CSB-MP347017HQN
CSB-EP347017HQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein ecsC (ecsC) CSB-YP347018BRJ
CSB-EP347018BRJ
CSB-BP347018BRJ
CSB-MP347018BRJ
CSB-EP347018BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4aF (NGR_a00420) CSB-YP347019RKX
CSB-EP347019RKX
CSB-BP347019RKX
CSB-MP347019RKX
CSB-EP347019RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4bD/y4pK (NGR_a00250) CSB-YP347020RKX
CSB-EP347020RKX
CSB-BP347020RKX
CSB-MP347020RKX
CSB-EP347020RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4bK (NGR_a00190) CSB-YP347021RKX
CSB-EP347021RKX
CSB-BP347021RKX
CSB-MP347021RKX
CSB-EP347021RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Uncharacterized protein y4cA (NGR_a00130), partial CSB-YP347022RKX
CSB-EP347022RKX
CSB-BP347022RKX
CSB-MP347022RKX
CSB-EP347022RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Probable cold shock protein y4cH (NGR_a00060) CSB-YP347023RKX
CSB-EP347023RKX
CSB-BP347023RKX
CSB-MP347023RKX
CSB-EP347023RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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