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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pasteurella multocida UvrABC system protein C (uvrC), partial CSB-YP348830ESG
CSB-EP348830ESG
CSB-BP348830ESG
CSB-MP348830ESG
CSB-EP348830ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP348831ESG
CSB-EP348831ESG
CSB-BP348831ESG
CSB-MP348831ESG
CSB-EP348831ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida DNA-binding protein fis (fis) CSB-YP348832ESG
CSB-EP348832ESG
CSB-BP348832ESG
CSB-MP348832ESG
CSB-EP348832ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida 30S ribosomal protein S18 (rpsR) CSB-YP348833ESG
CSB-EP348833ESG
CSB-BP348833ESG
CSB-MP348833ESG
CSB-EP348833ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Leucine--tRNA ligase (leuS), partial CSB-YP348834ESG
CSB-EP348834ESG
CSB-BP348834ESG
CSB-MP348834ESG
CSB-EP348834ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Alanine--tRNA ligase (alaS), partial CSB-YP348835ESG
CSB-EP348835ESG
CSB-BP348835ESG
CSB-MP348835ESG
CSB-EP348835ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP348836ESG
CSB-EP348836ESG
CSB-BP348836ESG
CSB-MP348836ESG
CSB-EP348836ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP348837ESG
CSB-EP348837ESG
CSB-BP348837ESG
CSB-MP348837ESG
CSB-EP348837ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Ribose-5-phosphate isomerase A (rpiA) CSB-YP348838ESG
CSB-EP348838ESG
CSB-BP348838ESG
CSB-MP348838ESG
CSB-EP348838ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida ATP-dependent protease subunit HslV (hslV) CSB-YP348839ESG
CSB-EP348839ESG
CSB-BP348839ESG
CSB-MP348839ESG
CSB-EP348839ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Phosphate acyltransferase (plsX) CSB-YP348840ESG
CSB-EP348840ESG
CSB-BP348840ESG
CSB-MP348840ESG
CSB-EP348840ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP348841ESG
CSB-EP348841ESG
CSB-BP348841ESG
CSB-MP348841ESG
CSB-EP348841ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable serine/threonine-protein kinase pknG (pknG), partial CSB-YP348842MVN
CSB-EP348842MVN
CSB-BP348842MVN
CSB-MP348842MVN
CSB-EP348842MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima Uncharacterized protein TM_0562.1 (TM_0562.1), partial CSB-YP348843TNJ1
CSB-EP348843TNJ1
CSB-BP348843TNJ1
CSB-MP348843TNJ1
CSB-EP348843TNJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yrhD (yrhD) CSB-YP348844ENV
CSB-EP348844ENV
CSB-BP348844ENV
CSB-MP348844ENV
CSB-EP348844ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP348845GFM
CSB-EP348845GFM
CSB-BP348845GFM
CSB-MP348845GFM
CSB-EP348845GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Probable protein-export membrane protein SecG (secG), partial CSB-YP348846MLN1
CSB-EP348846MLN1
CSB-BP348846MLN1
CSB-MP348846MLN1
CSB-EP348846MLN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin ParD (parD) CSB-YP348847EOD
CSB-EP348847EOD
CSB-BP348847EOD
CSB-MP348847EOD
CSB-EP348847EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Dephospho-CoA kinase (coaE) CSB-YP348848DQO
CSB-EP348848DQO
CSB-BP348848DQO
CSB-MP348848DQO
CSB-EP348848DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus kodakaraensis Transcription initiation factor IIB 2 (tfb2) CSB-YP348849FHY
CSB-EP348849FHY
CSB-BP348849FHY
CSB-MP348849FHY
CSB-EP348849FHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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