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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Cytosolic sulfotransferase 12 (SOT12) CSB-YP347147DOA
CSB-EP347147DOA
CSB-BP347147DOA
CSB-MP347147DOA
CSB-EP347147DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase III subunit beta (dnaN) CSB-YP347148MVW
CSB-EP347148MVW
CSB-BP347148MVW
CSB-MP347148MVW
CSB-EP347148MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S14 (rpsN) CSB-YP347149FPZ
CSB-EP347149FPZ
CSB-BP347149FPZ
CSB-MP347149FPZ
CSB-EP347149FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC23 (CWC23) CSB-YP347150SVG
CSB-EP347150SVG
CSB-BP347150SVG
CSB-MP347150SVG
CSB-EP347150SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein clpf-1 (clpf-1) CSB-YP347151CXY
CSB-EP347151CXY
CSB-BP347151CXY
CSB-MP347151CXY
CSB-EP347151CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein F46C5.7 (F46C5.7), partial CSB-YP347152CXY1
CSB-EP347152CXY1
CSB-BP347152CXY1
CSB-MP347152CXY1
CSB-EP347152CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Prostaglandin F synthase 2 CSB-YP347153BO
CSB-EP347153BO
CSB-BP347153BO
CSB-MP347153BO
CSB-EP347153BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial, partial CSB-YP347154EWE
CSB-EP347154EWE
CSB-BP347154EWE
CSB-MP347154EWE
CSB-EP347154EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein COS10 (COS10), partial CSB-YP347155SVG
CSB-EP347155SVG
CSB-BP347155SVG
CSB-MP347155SVG
CSB-EP347155SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Stage 0 sporulation protein A (spo0A) CSB-YP347156GFM
CSB-EP347156GFM
CSB-BP347156GFM
CSB-MP347156GFM
CSB-EP347156GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP347157FNW
CSB-EP347157FNW
CSB-BP347157FNW
CSB-MP347157FNW
CSB-EP347157FNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Homoserine dehydrogenase (hom) CSB-YP347158SSQ
CSB-EP347158SSQ
CSB-BP347158SSQ
CSB-MP347158SSQ
CSB-EP347158SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pantothenate synthetase (panC) CSB-YP347159BRJ
CSB-EP347159BRJ
CSB-BP347159BRJ
CSB-MP347159BRJ
CSB-EP347159BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable propionyl-CoA carboxylase beta chain 5 (accD5), partial CSB-YP347160MVN
CSB-EP347160MVN
CSB-BP347160MVN
CSB-MP347160MVN
CSB-EP347160MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major sperm protein 33 (msp-33) CSB-YP347161CXY
CSB-EP347161CXY
CSB-BP347161CXY
CSB-MP347161CXY
CSB-EP347161CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulatory protein MIG2 (MIG2) CSB-YP347162SVG
CSB-EP347162SVG
CSB-BP347162SVG
CSB-MP347162SVG
CSB-EP347162SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein transport protein YIP1 (YIP1), partial CSB-YP347163SVG1
CSB-EP347163SVG1
CSB-BP347163SVG1
CSB-MP347163SVG1
CSB-EP347163SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein VEL1 (VEL1) CSB-YP347164SVG
CSB-EP347164SVG
CSB-BP347164SVG
CSB-MP347164SVG
CSB-EP347164SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Anaphase-promoting complex subunit DOC1 (DOC1) CSB-YP347165SVG
CSB-EP347165SVG
CSB-BP347165SVG
CSB-MP347165SVG
CSB-EP347165SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sensitivity to high expression protein 10 (SHE10), partial CSB-YP347166SVG
CSB-EP347166SVG
CSB-BP347166SVG
CSB-MP347166SVG
CSB-EP347166SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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