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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Flagellar hook protein flgE (flgE) CSB-YP348141BWZ
CSB-EP348141BWZ
CSB-BP348141BWZ
CSB-MP348141BWZ
CSB-EP348141BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ribosomal large subunit pseudouridine synthase C (rluC) CSB-YP348142BWZ
CSB-EP348142BWZ
CSB-BP348142BWZ
CSB-MP348142BWZ
CSB-EP348142BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Uncharacterized protein BU358 (BU358) CSB-YP348143BWZ
CSB-EP348143BWZ
CSB-BP348143BWZ
CSB-MP348143BWZ
CSB-EP348143BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Valine--tRNA ligase (valS), partial CSB-YP348144BWZ
CSB-EP348144BWZ
CSB-BP348144BWZ
CSB-MP348144BWZ
CSB-EP348144BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 30S ribosomal protein S15 (rpsO) CSB-YP348145BWZ
CSB-EP348145BWZ
CSB-BP348145BWZ
CSB-MP348145BWZ
CSB-EP348145BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP348146BWZ
CSB-EP348146BWZ
CSB-BP348146BWZ
CSB-MP348146BWZ
CSB-EP348146BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 50S ribosomal protein L13 (rplM) CSB-YP348147BWZ
CSB-EP348147BWZ
CSB-BP348147BWZ
CSB-MP348147BWZ
CSB-EP348147BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Protein tldD homolog (tldD) CSB-YP348148BWZ
CSB-EP348148BWZ
CSB-BP348148BWZ
CSB-MP348148BWZ
CSB-EP348148BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Glutamate--cysteine ligase (gshA), partial CSB-YP348149BWZ
CSB-EP348149BWZ
CSB-BP348149BWZ
CSB-MP348149BWZ
CSB-EP348149BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Protein NlpD/LppB homolog (BU418) CSB-YP348150BWZ
CSB-EP348150BWZ
CSB-BP348150BWZ
CSB-MP348150BWZ
CSB-EP348150BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Siroheme synthase (cysG) CSB-YP348151BWZ
CSB-EP348151BWZ
CSB-BP348151BWZ
CSB-MP348151BWZ
CSB-EP348151BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Probable rRNA maturation factor (BU442) CSB-YP348152BWZ
CSB-EP348152BWZ
CSB-BP348152BWZ
CSB-MP348152BWZ
CSB-EP348152BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Phosphoglycerate kinase (pgk) CSB-YP348153BWZ
CSB-EP348153BWZ
CSB-BP348153BWZ
CSB-MP348153BWZ
CSB-EP348153BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Diaminohydroxyphosphoribosylamino-pyrimidine deaminase (ribD1) CSB-YP348154BWZ
CSB-EP348154BWZ
CSB-BP348154BWZ
CSB-MP348154BWZ
CSB-EP348154BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Cytochrome o ubiquinol oxidase protein CyoD (cyoD), partial CSB-YP348155BWZ1
CSB-EP348155BWZ1
CSB-BP348155BWZ1
CSB-MP348155BWZ1
CSB-EP348155BWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP348156BWZ
CSB-EP348156BWZ
CSB-BP348156BWZ
CSB-MP348156BWZ
CSB-EP348156BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum UPF0133 protein BU482 (BU482) CSB-YP348157BWZ
CSB-EP348157BWZ
CSB-BP348157BWZ
CSB-MP348157BWZ
CSB-EP348157BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Protein smg (smg) CSB-YP348158BWZ
CSB-EP348158BWZ
CSB-BP348158BWZ
CSB-MP348158BWZ
CSB-EP348158BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 30S ribosomal protein S13 (rpsM) CSB-YP348159BWZ
CSB-EP348159BWZ
CSB-BP348159BWZ
CSB-MP348159BWZ
CSB-EP348159BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 50S ribosomal protein L5 (rplE) CSB-YP348160BWZ
CSB-EP348160BWZ
CSB-BP348160BWZ
CSB-MP348160BWZ
CSB-EP348160BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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