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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Syntaxin-81 (SYP81), partial CSB-YP348545DOA
CSB-EP348545DOA
CSB-BP348545DOA
CSB-MP348545DOA
CSB-EP348545DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Ureidoglycolate hydrolase (allA) CSB-YP348546BMQ
CSB-EP348546BMQ
CSB-BP348546BMQ
CSB-MP348546BMQ
CSB-EP348546BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative mannosyl-3-phosphoglycerate phosphatase (yedP) CSB-YP348547EGX
CSB-EP348547EGX
CSB-BP348547EGX
CSB-MP348547EGX
CSB-EP348547EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 60S ribosomal protein L37-A (rpl37a) CSB-YP348548SXV
CSB-EP348548SXV
CSB-BP348548SXV
CSB-MP348548SXV
CSB-EP348548SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Metallothiol transferase fosB (fosB) CSB-YP348549SLD
CSB-EP348549SLD
CSB-BP348549SLD
CSB-MP348549SLD
CSB-EP348549SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Amino-acid acetyltransferase (argA) CSB-YP348550SZB
CSB-EP348550SZB
CSB-BP348550SZB
CSB-MP348550SZB
CSB-EP348550SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Acetylglutamate kinase (argB) CSB-YP348551BGP
CSB-EP348551BGP
CSB-BP348551BGP
CSB-MP348551BGP
CSB-EP348551BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Acetylglutamate kinase (argB) CSB-YP348552DST
CSB-EP348552DST
CSB-BP348552DST
CSB-MP348552DST
CSB-EP348552DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Acetylglutamate kinase (argB) CSB-YP348553LOS
CSB-EP348553LOS
CSB-BP348553LOS
CSB-MP348553LOS
CSB-EP348553LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Acetylglutamate kinase (argB) CSB-YP348554SGA
CSB-EP348554SGA
CSB-BP348554SGA
CSB-MP348554SGA
CSB-EP348554SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Acetylglutamate kinase (argB) CSB-YP348555TDQ
CSB-EP348555TDQ
CSB-BP348555TDQ
CSB-MP348555TDQ
CSB-EP348555TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP348556BGP
CSB-EP348556BGP
CSB-BP348556BGP
CSB-MP348556BGP
CSB-EP348556BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP348557LOS
CSB-EP348557LOS
CSB-BP348557LOS
CSB-MP348557LOS
CSB-EP348557LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP348558SGA
CSB-EP348558SGA
CSB-BP348558SGA
CSB-MP348558SGA
CSB-EP348558SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP348559FMR
CSB-EP348559FMR
CSB-BP348559FMR
CSB-MP348559FMR
CSB-EP348559FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP348560VFI
CSB-EP348560VFI
CSB-BP348560VFI
CSB-MP348560VFI
CSB-EP348560VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Acetylornithine aminotransferase (argD) CSB-YP348561BGP
CSB-EP348561BGP
CSB-BP348561BGP
CSB-MP348561BGP
CSB-EP348561BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylornithine/succinyldiaminopimelate aminotransferase (argD) CSB-YP348562EGX
CSB-EP348562EGX
CSB-BP348562EGX
CSB-MP348562EGX
CSB-EP348562EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Acetylornithine aminotransferase (argD) CSB-YP348563FGB
CSB-EP348563FGB
CSB-BP348563FGB
CSB-MP348563FGB
CSB-EP348563FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylornithine/succinyldiaminopimelate aminotransferase (argD) CSB-YP348564SZB
CSB-EP348564SZB
CSB-BP348564SZB
CSB-MP348564SZB
CSB-EP348564SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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