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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Periplasmic protein torT (torT) CSB-YP348871EOD
CSB-EP348871EOD
CSB-BP348871EOD
CSB-MP348871EOD
CSB-EP348871EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus tokodaii 50S ribosomal protein L30e (rpl30e) CSB-YP348872FPN
CSB-EP348872FPN
CSB-BP348872FPN
CSB-MP348872FPN
CSB-EP348872FPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication terminus site-binding protein (tus) CSB-YP348873EOD
CSB-EP348873EOD
CSB-BP348873EOD
CSB-MP348873EOD
CSB-EP348873EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureidoglycolate dehydrogenase (allD) CSB-YP348874EOD
CSB-EP348874EOD
CSB-BP348874EOD
CSB-MP348874EOD
CSB-EP348874EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fructose-6-phosphate aldolase 2 (fsaB) CSB-YP348875EOD
CSB-EP348875EOD
CSB-BP348875EOD
CSB-MP348875EOD
CSB-EP348875EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus tokodaii Sugar fermentation stimulation protein homolog (sfsA) CSB-YP348876FPN
CSB-EP348876FPN
CSB-BP348876FPN
CSB-MP348876FPN
CSB-EP348876FPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Arginine--tRNA ligase (argS), partial CSB-YP348877HUV
CSB-EP348877HUV
CSB-BP348877HUV
CSB-MP348877HUV
CSB-EP348877HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Tryptophan synthase beta chain (trpB) CSB-YP348878HUV
CSB-EP348878HUV
CSB-BP348878HUV
CSB-MP348878HUV
CSB-EP348878HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II 12 kDa extrinsic protein (psbU) CSB-YP348879TNI
CSB-EP348879TNI
CSB-BP348879TNI
CSB-MP348879TNI
CSB-EP348879TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP348880HUV
CSB-EP348880HUV
CSB-BP348880HUV
CSB-MP348880HUV
CSB-EP348880HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phalaris aquatica Major pollen allergen Pha a 5.2 CSB-YP348881PGU
CSB-EP348881PGU
CSB-BP348881PGU
CSB-MP348881PGU
CSB-EP348881PGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Bifunctional NAD (P)H-hydrate repair enzyme Nnr CSB-YP348882HUV
CSB-EP348882HUV
CSB-BP348882HUV
CSB-MP348882HUV
CSB-EP348882HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribose-phosphate pyrophosphokinase (prs) CSB-YP348883HUV
CSB-EP348883HUV
CSB-BP348883HUV
CSB-MP348883HUV
CSB-EP348883HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phosphopentomutase (deoB) CSB-YP348884HUV
CSB-EP348884HUV
CSB-BP348884HUV
CSB-MP348884HUV
CSB-EP348884HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cAMP-regulated phosphoprotein 19 (ARPP19) CSB-YP348885HU
CSB-EP348885HU
CSB-BP348885HU
CSB-MP348885HU
CSB-EP348885HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus serrulatus Potassium channel toxin alpha-KTx 4.2 CSB-YP348886TON
CSB-EP348886TON
CSB-BP348886TON
CSB-MP348886TON
CSB-EP348886TON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria caerulea Caerin-1.3 CSB-YP348887LHF
CSB-EP348887LHF
CSB-BP348887LHF
CSB-MP348887LHF
CSB-EP348887LHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria caerulea Caerin-3.4 CSB-YP348888LHF
CSB-EP348888LHF
CSB-BP348888LHF
CSB-MP348888LHF
CSB-EP348888LHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-alpha-L-fucosyltransferase (wecF) CSB-YP348889ENV
CSB-EP348889ENV
CSB-BP348889ENV
CSB-MP348889ENV
CSB-EP348889ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen fixation regulatory protein (nifL) CSB-YP348890KBE
CSB-EP348890KBE
CSB-BP348890KBE
CSB-MP348890KBE
CSB-EP348890KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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