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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Acetylornithine/succinyldiaminopimelate aminotransferase (argD) CSB-YP349420YAS
CSB-EP349420YAS
CSB-BP349420YAS
CSB-MP349420YAS
CSB-EP349420YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-cysteine desulfhydrase (dcyD) CSB-YP349421SZB
CSB-EP349421SZB
CSB-BP349421SZB
CSB-MP349421SZB
CSB-EP349421SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molecular chaperone Hsp31 and glyoxalase 3 (hchA) CSB-YP349422SZB
CSB-EP349422SZB
CSB-BP349422SZB
CSB-MP349422SZB
CSB-EP349422SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus sp. L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP349423PSQ
CSB-EP349423PSQ
CSB-BP349423PSQ
CSB-MP349423PSQ
CSB-EP349423PSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus sp. Haloalkane dehalogenase (dhaA) CSB-YP349424RDM
CSB-EP349424RDM
CSB-BP349424RDM
CSB-MP349424RDM
CSB-EP349424RDM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulatory protein dcuR (dcuR) CSB-YP349425EGX
CSB-EP349425EGX
CSB-BP349425EGX
CSB-MP349425EGX
CSB-EP349425EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sensor histidine kinase DcuS (dcuS), partial CSB-YP349426EGX1
CSB-EP349426EGX1
CSB-BP349426EGX1
CSB-MP349426EGX1
CSB-EP349426EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal transduction histidine-protein kinase BarA (barA), partial CSB-YP349427SZB
CSB-EP349427SZB
CSB-BP349427SZB
CSB-MP349427SZB
CSB-EP349427SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Disulfide bond formation protein B 2 (dsbB2), partial CSB-YP349428FGB1
CSB-EP349428FGB1
CSB-BP349428FGB1
CSB-MP349428FGB1
CSB-EP349428FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Disulfide bond formation protein B (dsbB), partial CSB-YP349429SGA1
CSB-EP349429SGA1
CSB-BP349429SGA1
CSB-MP349429SGA1
CSB-EP349429SGA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0229 protein bll6755 (bll6755) CSB-YP349430BVW
CSB-EP349430BVW
CSB-BP349430BVW
CSB-MP349430BVW
CSB-EP349430BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0229 protein OB2647 (OB2647) CSB-YP349431OAB
CSB-EP349431OAB
CSB-BP349431OAB
CSB-MP349431OAB
CSB-EP349431OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0229 protein SO_2883 (SO_2883) CSB-YP349432SGA
CSB-EP349432SGA
CSB-BP349432SGA
CSB-MP349432SGA
CSB-EP349432SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buthus occitanus mardochei Alpha-like toxin Bom4 CSB-YP349433BOB
CSB-EP349433BOB
CSB-BP349433BOB
CSB-MP349433BOB
CSB-EP349433BOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Alpha-mammal toxin Lqh2 CSB-YP349434LDS
CSB-EP349434LDS
CSB-BP349434LDS
CSB-MP349434LDS
CSB-EP349434LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoradendron liga Ligatoxin-B CSB-YP349435PHY
CSB-EP349435PHY
CSB-BP349435PHY
CSB-MP349435PHY
CSB-EP349435PHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Neurotoxin BmK-II CSB-YP349436MRE
CSB-EP349436MRE
CSB-BP349436MRE
CSB-MP349436MRE
CSB-EP349436MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Excinuclease cho (cho) CSB-YP349437SZB
CSB-EP349437SZB
CSB-BP349437SZB
CSB-MP349437SZB
CSB-EP349437SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable intracellular septation protein A (PP_4501), partial CSB-YP349438FGB1
CSB-EP349438FGB1
CSB-BP349438FGB1
CSB-MP349438FGB1
CSB-EP349438FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable intracellular septation protein A (VV1_3073), partial CSB-YP349439VFI1
CSB-EP349439VFI1
CSB-BP349439VFI1
CSB-MP349439VFI1
CSB-EP349439VFI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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