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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Deoxyribose-phosphate aldolase (deoC) CSB-YP350642MVZ
CSB-EP350642MVZ
CSB-BP350642MVZ
CSB-MP350642MVZ
CSB-EP350642MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Deoxyguanosinetriphosphate triphosphohydrolase-like protein (BMEI1090) CSB-YP350643BMO
CSB-EP350643BMO
CSB-BP350643BMO
CSB-MP350643BMO
CSB-EP350643BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate synthase (dapA) CSB-YP350644MVH
CSB-EP350644MVH
CSB-BP350644MVH
CSB-MP350644MVH
CSB-EP350644MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol dehydrogenase (hisD) CSB-YP350645MVZ
CSB-EP350645MVZ
CSB-BP350645MVZ
CSB-MP350645MVZ
CSB-EP350645MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidinol dehydrogenase (hisD) CSB-YP350646SUL
CSB-EP350646SUL
CSB-BP350646SUL
CSB-MP350646SUL
CSB-EP350646SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP350647FMW
CSB-EP350647FMW
CSB-BP350647FMW
CSB-MP350647FMW
CSB-EP350647FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA primase (dnaG), partial CSB-YP350648SKX
CSB-EP350648SKX
CSB-BP350648SKX
CSB-MP350648SKX
CSB-EP350648SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ 2 (dnaJ2) CSB-YP350649MVZ
CSB-EP350649MVZ
CSB-BP350649MVZ
CSB-MP350649MVZ
CSB-EP350649MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Chaperone protein DnaJ (dnaJ) CSB-YP350650NGG
CSB-EP350650NGG
CSB-BP350650NGG
CSB-MP350650NGG
CSB-EP350650NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP350651MVZ
CSB-EP350651MVZ
CSB-BP350651MVZ
CSB-MP350651MVZ
CSB-EP350651MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III polC-type (polC), partial CSB-YP350652SKY
CSB-EP350652SKY
CSB-BP350652SKY
CSB-MP350652SKY
CSB-EP350652SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase IV 1 (dinB1) CSB-YP350653MVH
CSB-EP350653MVH
CSB-BP350653MVH
CSB-MP350653MVH
CSB-EP350653MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase IV (dinB) CSB-YP350654SWW
CSB-EP350654SWW
CSB-BP350654SWW
CSB-MP350654SWW
CSB-EP350654SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Disulfide bond formation protein B (dsbB), partial CSB-YP350655SXB1
CSB-EP350655SXB1
CSB-BP350655SXB1
CSB-MP350655SXB1
CSB-EP350655SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Elongation factor P (efp) CSB-YP350658LPY
CSB-EP350658LPY
CSB-BP350658LPY
CSB-MP350658LPY
CSB-EP350658LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Elongation factor P (efp) CSB-YP350659LGU
CSB-EP350659LGU
CSB-BP350659LGU
CSB-MP350659LGU
CSB-EP350659LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor P (efp) CSB-YP350660SUL
CSB-EP350660SUL
CSB-BP350660SUL
CSB-MP350660SUL
CSB-EP350660SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP350661SMH
CSB-EP350661SMH
CSB-BP350661SMH
CSB-MP350661SMH
CSB-EP350661SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP350662SMI
CSB-EP350662SMI
CSB-BP350662SMI
CSB-MP350662SMI
CSB-EP350662SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Elongation factor P (efp) CSB-YP350663SMP
CSB-EP350663SMP
CSB-BP350663SMP
CSB-MP350663SMP
CSB-EP350663SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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