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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neisseria meningitidis serogroup A / serotype 4A Putative bacterioferritin B (bfrB) CSB-YP350602NGF
CSB-EP350602NGF
CSB-BP350602NGF
CSB-MP350602NGF
CSB-EP350602NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose 1,6-diphosphate aldolase 1 (lacD1) CSB-YP350603SMT
CSB-EP350603SMT
CSB-BP350603SMT
CSB-MP350603SMT
CSB-EP350603SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP350604SMH
CSB-EP350604SMH
CSB-BP350604SMH
CSB-MP350604SMH
CSB-EP350604SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Carbamoyl-phosphate synthase small chain (carA) CSB-YP350605SMP
CSB-EP350605SMP
CSB-BP350605SMP
CSB-MP350605SMP
CSB-EP350605SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP350606SKX
CSB-EP350606SKX
CSB-BP350606SKX
CSB-MP350606SKX
CSB-EP350606SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ESX-5 secretion system protein EccA5 (eccA5), partial CSB-YP350607MVZ
CSB-EP350607MVZ
CSB-BP350607MVZ
CSB-MP350607MVZ
CSB-EP350607MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ethanolamine utilization protein EutS (eutS) CSB-YP350608EGX
CSB-EP350608EGX
CSB-BP350608EGX
CSB-MP350608EGX
CSB-EP350608EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP350609MVZ1
CSB-EP350609MVZ1
CSB-BP350609MVZ1
CSB-MP350609MVZ1
CSB-EP350609MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase 1 (pgsA1), partial CSB-YP350610MVZ1
CSB-EP350610MVZ1
CSB-BP350610MVZ1
CSB-MP350610MVZ1
CSB-EP350610MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized FtsW-like protein (rodA), partial CSB-YP350611MVZ
CSB-EP350611MVZ
CSB-BP350611MVZ
CSB-MP350611MVZ
CSB-EP350611MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell division protein FtsA (ftsA) CSB-YP350612SKY
CSB-EP350612SKY
CSB-BP350612SKY
CSB-MP350612SKY
CSB-EP350612SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP350613SMH
CSB-EP350613SMH
CSB-BP350613SMH
CSB-MP350613SMH
CSB-EP350613SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP350614XBL
CSB-EP350614XBL
CSB-BP350614XBL
CSB-MP350614XBL
CSB-EP350614XBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP350615SKX
CSB-EP350615SKX
CSB-BP350615SKX
CSB-MP350615SKX
CSB-EP350615SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP350616SMT
CSB-EP350616SMT
CSB-BP350616SMT
CSB-MP350616SMT
CSB-EP350616SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP350617SKY
CSB-EP350617SKY
CSB-BP350617SKY
CSB-MP350617SKY
CSB-EP350617SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cytidylate kinase (cmk) CSB-YP350618SKY
CSB-EP350618SKY
CSB-BP350618SKY
CSB-MP350618SKY
CSB-EP350618SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantothenate kinase (coaA) CSB-YP350619MVZ
CSB-EP350619MVZ
CSB-BP350619MVZ
CSB-MP350619MVZ
CSB-EP350619MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantothenate kinase (coaA) CSB-YP350620SMI
CSB-EP350620SMI
CSB-BP350620SMI
CSB-MP350620SMI
CSB-EP350620SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Phosphopantetheine adenylyltransferase (coaD) CSB-YP350621NGG
CSB-EP350621NGG
CSB-BP350621NGG
CSB-MP350621NGG
CSB-EP350621NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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