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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Anti-sigma-B factor antagonist (rsbV) CSB-YP351472FLF
CSB-EP351472FLF
CSB-BP351472FLF
CSB-MP351472FLF
CSB-EP351472FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0291 protein MW2494 (MW2494) CSB-YP351473SUL
CSB-EP351473SUL
CSB-BP351473SUL
CSB-MP351473SUL
CSB-EP351473SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginase (arg) CSB-YP351474SKX
CSB-EP351474SKX
CSB-BP351474SKX
CSB-MP351474SKX
CSB-EP351474SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal protein L11 methyltransferase (prmA) CSB-YP351475VCQ
CSB-EP351475VCQ
CSB-BP351475VCQ
CSB-MP351475VCQ
CSB-EP351475VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L11 (rplK) CSB-YP351476CKA
CSB-EP351476CKA
CSB-BP351476CKA
CSB-MP351476CKA
CSB-EP351476CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus TelA-like protein SA1238 (SA1238) CSB-YP351477SKY
CSB-EP351477SKY
CSB-BP351477SKY
CSB-MP351477SKY
CSB-EP351477SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera biennis ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP351478ODZ1
CSB-EP351478ODZ1
CSB-BP351478ODZ1
CSB-MP351478ODZ1
CSB-EP351478ODZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saururus cernuus Photosystem II reaction center protein L (psbL), partial CSB-YP351479SDB
CSB-EP351479SDB
CSB-BP351479SDB
CSB-MP351479SDB
CSB-EP351479SDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Photosystem II reaction center protein L (psbL), partial CSB-YP351480TQN
CSB-EP351480TQN
CSB-BP351480TQN
CSB-MP351480TQN
CSB-EP351480TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tomentosiformis Photosystem II reaction center protein L (psbL), partial CSB-YP351481NDO
CSB-EP351481NDO
CSB-BP351481NDO
CSB-MP351481NDO
CSB-EP351481NDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psilotum nudum Photosystem II reaction center protein L (psbL), partial CSB-YP351482FGY
CSB-EP351482FGY
CSB-BP351482FGY
CSB-MP351482FGY
CSB-EP351482FGY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transglycosylase isaA (isaA) CSB-YP351483FKZ
CSB-EP351483FKZ
CSB-BP351483FKZ
CSB-MP351483FKZ
CSB-EP351483FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides gracilis Toxin Cg2 CSB-YP351484CFX
CSB-EP351484CFX
CSB-BP351484CFX
CSB-MP351484CFX
CSB-EP351484CFX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Measles virus Non-structural protein V (P/V) CSB-YP351485MCZ
CSB-EP351485MCZ
CSB-BP351485MCZ
CSB-MP351485MCZ
CSB-EP351485MCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adapter protein mecA (mecA) CSB-YP351486SMU
CSB-EP351486SMU
CSB-BP351486SMU
CSB-MP351486SMU
CSB-EP351486SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ribonuclease BN (rbn) CSB-YP351487SXB
CSB-EP351487SXB
CSB-BP351487SXB
CSB-MP351487SXB
CSB-EP351487SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus obscurus Potassium channel toxin alpha-KTx 4.4 CSB-YP351488TET
CSB-EP351488TET
CSB-BP351488TET
CSB-MP351488TET
CSB-EP351488TET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Thioredoxin-1 (dhd) CSB-YP351490DMR
CSB-EP351490DMR
CSB-BP351490DMR
CSB-MP351490DMR
CSB-EP351490DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Muscarinic m1-toxin2 CSB-YP351491DBG
CSB-EP351491DBG
CSB-BP351491DBG
CSB-MP351491DBG
CSB-EP351491DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis F420-non-reducing hydrogenase subunit G (mvhG) CSB-YP351492MSQ
CSB-EP351492MSQ
CSB-BP351492MSQ
CSB-MP351492MSQ
CSB-EP351492MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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