Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant ATP phosphoribosyltransferase (hisG) CSB-YP351554EGX
CSB-EP351554EGX
CSB-BP351554EGX
CSB-MP351554EGX
CSB-EP351554EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda semifasciata Erabutoxin a (ETXA) CSB-YP351555LDC
CSB-EP351555LDC
CSB-BP351555LDC
CSB-MP351555LDC
CSB-EP351555LDC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP351556ENV1
CSB-EP351556ENV1
CSB-BP351556ENV1
CSB-MP351556ENV1
CSB-EP351556ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Elongation factor 4 (lepA), partial CSB-YP351557GBK
CSB-EP351557GBK
CSB-BP351557GBK
CSB-MP351557GBK
CSB-EP351557GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP351558MVH
CSB-EP351558MVH
CSB-BP351558MVH
CSB-MP351558MVH
CSB-EP351558MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP351559SXB
CSB-EP351559SXB
CSB-BP351559SXB
CSB-MP351559SXB
CSB-EP351559SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Foldase protein prsA 1 (prsA1) CSB-YP351560SMT
CSB-EP351560SMT
CSB-BP351560SMT
CSB-MP351560SMT
CSB-EP351560SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0305 protein MMP0665 (MMP0665) CSB-YP351561MSB
CSB-EP351561MSB
CSB-BP351561MSB
CSB-MP351561MSB
CSB-EP351561MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease P protein component 1 (rnp1) CSB-YP351562MSB
CSB-EP351562MSB
CSB-BP351562MSB
CSB-MP351562MSB
CSB-EP351562MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At1g12290 (At1g12290), partial CSB-YP351563DOA
CSB-EP351563DOA
CSB-BP351563DOA
CSB-MP351563DOA
CSB-EP351563DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L4P (rpl4p) CSB-YP351564MSB
CSB-EP351564MSB
CSB-BP351564MSB
CSB-MP351564MSB
CSB-EP351564MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Histidinol dehydrogenase (hisD) CSB-YP351565RLU
CSB-EP351565RLU
CSB-BP351565RLU
CSB-MP351565RLU
CSB-EP351565RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative transport protein YidE (yidE), partial CSB-YP351566SZB
CSB-EP351566SZB
CSB-BP351566SZB
CSB-MP351566SZB
CSB-EP351566SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP351567CQF
CSB-EP351567CQF
CSB-BP351567CQF
CSB-MP351567CQF
CSB-EP351567CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP351568FKZ
CSB-EP351568FKZ
CSB-BP351568FKZ
CSB-MP351568FKZ
CSB-EP351568FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Histidine--tRNA ligase (hisS) CSB-YP351569OBN
CSB-EP351569OBN
CSB-BP351569OBN
CSB-MP351569OBN
CSB-EP351569OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Aquaporin Z (aqpZ), partial CSB-YP351570RLU1
CSB-EP351570RLU1
CSB-BP351570RLU1
CSB-MP351570RLU1
CSB-EP351570RLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Undecaprenyl-diphosphatase (uppP), partial CSB-YP351571ENV1
CSB-EP351571ENV1
CSB-BP351571ENV1
CSB-MP351571ENV1
CSB-EP351571ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP351572MLA
CSB-EP351572MLA
CSB-BP351572MLA
CSB-MP351572MLA
CSB-EP351572MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribose uptake protein rbsU (rbsU), partial CSB-YP351573SKX
CSB-EP351573SKX
CSB-BP351573SKX
CSB-MP351573SKX
CSB-EP351573SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>