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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nanoarchaeum equitans UPF0215 protein NEQ431 (NEQ431) CSB-YP351595NAQ
CSB-EP351595NAQ
CSB-BP351595NAQ
CSB-MP351595NAQ
CSB-EP351595NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L22 (rplV) CSB-YP351596ENV
CSB-EP351596ENV
CSB-BP351596ENV
CSB-MP351596ENV
CSB-EP351596ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP351597MVZ
CSB-EP351597MVZ
CSB-BP351597MVZ
CSB-MP351597MVZ
CSB-EP351597MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein htpG (htpG), partial CSB-YP351598MLA
CSB-EP351598MLA
CSB-BP351598MLA
CSB-MP351598MLA
CSB-EP351598MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Methylaspartate mutase S chain (mamA) CSB-YP351599TPP
CSB-EP351599TPP
CSB-BP351599TPP
CSB-MP351599TPP
CSB-EP351599TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus DNA-directed RNA polymerases I, II, and III subunit RPABC2 (POLR2F) CSB-YP351600DXU
CSB-EP351600DXU
CSB-BP351600DXU
CSB-MP351600DXU
CSB-EP351600DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrothele raveni Beta-hexatoxin-Mr1a CSB-YP351601MAN
CSB-EP351601MAN
CSB-BP351601MAN
CSB-MP351601MAN
CSB-EP351601MAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi 30S ribosomal protein S27ae (rps27ae) CSB-YP351602FHV
CSB-EP351602FHV
CSB-BP351602FHV
CSB-MP351602FHV
CSB-EP351602FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Protein ycf2 (ycf2), partial CSB-YP351603EVD
CSB-EP351603EVD
CSB-BP351603EVD
CSB-MP351603EVD
CSB-EP351603EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Melanotropin alpha CSB-YP351604HO
CSB-EP351604HO
CSB-BP351604HO
CSB-MP351604HO
CSB-EP351604HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachemys scripta Somatostatin-14 (SST) CSB-YP351605THC
CSB-EP351605THC
CSB-BP351605THC
CSB-MP351605THC
CSB-EP351605THC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein carrier protein (lolA) CSB-YP351606EGX
CSB-EP351606EGX
CSB-BP351606EGX
CSB-MP351606EGX
CSB-EP351606EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein carrier protein (lolA) CSB-YP351607SZB
CSB-EP351607SZB
CSB-BP351607SZB
CSB-MP351607SZB
CSB-EP351607SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii HPr kinase/phosphorylase (hprK) CSB-YP351608LAW
CSB-EP351608LAW
CSB-BP351608LAW
CSB-MP351608LAW
CSB-EP351608LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP351609MLS
CSB-EP351609MLS
CSB-BP351609MLS
CSB-MP351609MLS
CSB-EP351609MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP351610WAQ
CSB-EP351610WAQ
CSB-BP351610WAQ
CSB-MP351610WAQ
CSB-EP351610WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 type 3 (rpmG3) CSB-YP351611FMW
CSB-EP351611FMW
CSB-BP351611FMW
CSB-MP351611FMW
CSB-EP351611FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 type 3 (rpmG3) CSB-YP351612SMI
CSB-EP351612SMI
CSB-BP351612SMI
CSB-MP351612SMI
CSB-EP351612SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate--cysteine ligase (gshA), partial CSB-YP351613PWD
CSB-EP351613PWD
CSB-BP351613PWD
CSB-MP351613PWD
CSB-EP351613PWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein CrcB homolog 1 (crcB1), partial CSB-YP351614SKY1
CSB-EP351614SKY1
CSB-BP351614SKY1
CSB-MP351614SKY1
CSB-EP351614SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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