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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Alanine--tRNA ligase (alaS), partial CSB-YP352242LEV
CSB-EP352242LEV
CSB-BP352242LEV
CSB-MP352242LEV
CSB-EP352242LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Alanine--tRNA ligase (alaS), partial CSB-YP352243TPP
CSB-EP352243TPP
CSB-BP352243TPP
CSB-MP352243TPP
CSB-EP352243TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP352244SZB
CSB-EP352244SZB
CSB-BP352244SZB
CSB-MP352244SZB
CSB-EP352244SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP352245GBK
CSB-EP352245GBK
CSB-BP352245GBK
CSB-MP352245GBK
CSB-EP352245GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 6,7-dimethyl-8-ribityllumazine synthase 2 (ribH2) CSB-YP352246RLU
CSB-EP352246RLU
CSB-BP352246RLU
CSB-MP352246RLU
CSB-EP352246RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium paratuberculosis Putative pterin-4-alpha-carbinolamine dehydratase (MAP_2623) CSB-YP352247MLA
CSB-EP352247MLA
CSB-BP352247MLA
CSB-MP352247MLA
CSB-EP352247MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP352248BQN
CSB-EP352248BQN
CSB-BP352248BQN
CSB-MP352248BQN
CSB-EP352248BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP352249LEV
CSB-EP352249LEV
CSB-BP352249LEV
CSB-MP352249LEV
CSB-EP352249LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP352250RLU
CSB-EP352250RLU
CSB-BP352250RLU
CSB-MP352250RLU
CSB-EP352250RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP352251DDH
CSB-EP352251DDH
CSB-BP352251DDH
CSB-MP352251DDH
CSB-EP352251DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Imidazole glycerol phosphate synthase subunit HisH 2 (hisH2) CSB-YP352252MSB
CSB-EP352252MSB
CSB-BP352252MSB
CSB-MP352252MSB
CSB-EP352252MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Precorrin-2 dehydrogenase (sirC) CSB-YP352253BQV
CSB-EP352253BQV
CSB-BP352253BQV
CSB-MP352253BQV
CSB-EP352253BQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bursal disease virus Structural polyprotein, partial CSB-YP352254ASD
CSB-EP352254ASD
CSB-BP352254ASD
CSB-MP352254ASD
CSB-EP352254ASD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Histone H3 (HHT1) CSB-YP352255SVG
CSB-EP352255SVG
CSB-BP352255SVG
CSB-MP352255SVG
CSB-EP352255SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aquaporin PIP1-1 (PIP1-1), partial CSB-YP352256DOA1
CSB-EP352256DOA1
CSB-BP352256DOA1
CSB-MP352256DOA1
CSB-EP352256DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem I assembly protein ycf3 (ycf3) CSB-YP352257DOA
CSB-EP352257DOA
CSB-BP352257DOA
CSB-MP352257DOA
CSB-EP352257DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba 50S ribosomal protein L23, chloroplastic (rpl23) CSB-YP352258SZO
CSB-EP352258SZO
CSB-BP352258SZO
CSB-MP352258SZO
CSB-EP352258SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protophormia terraenovae Adipokinetic hormone (AKH) CSB-YP352259PST
CSB-EP352259PST
CSB-BP352259PST
CSB-MP352259PST
CSB-EP352259PST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila hydei Tubulin beta-2 chain (betaTub85D) CSB-YP352260DLM
CSB-EP352260DLM
CSB-BP352260DLM
CSB-MP352260DLM
CSB-EP352260DLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mono- and diacylglycerol lipase (mdlA) CSB-YP352261PET
CSB-EP352261PET
CSB-BP352261PET
CSB-MP352261PET
CSB-EP352261PET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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