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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O157:H7 Flavodoxin-1 (fldA) CSB-YP352852EOD
CSB-EP352852EOD
CSB-BP352852EOD
CSB-MP352852EOD
CSB-EP352852EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP352853DDH
CSB-EP352853DDH
CSB-BP352853DDH
CSB-MP352853DDH
CSB-EP352853DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP352854MSB
CSB-EP352854MSB
CSB-BP352854MSB
CSB-MP352854MSB
CSB-EP352854MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus 30S ribosomal protein S4P (rps4p) CSB-YP352855FHW
CSB-EP352855FHW
CSB-BP352855FHW
CSB-MP352855FHW
CSB-EP352855FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii 50S ribosomal protein L37e (rpl37e) CSB-YP352856FHX
CSB-EP352856FHX
CSB-BP352856FHX
CSB-MP352856FHX
CSB-EP352856FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi 30S ribosomal protein S11P (rps11p) CSB-YP352857FHV
CSB-EP352857FHV
CSB-BP352857FHV
CSB-MP352857FHV
CSB-EP352857FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii DNA-directed RNA polymerase subunit K (rpoK) CSB-YP352858FHX
CSB-EP352858FHX
CSB-BP352858FHX
CSB-MP352858FHX
CSB-EP352858FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP352859BQN
CSB-EP352859BQN
CSB-BP352859BQN
CSB-MP352859BQN
CSB-EP352859BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable transcriptional regulatory protein BCE_0595 (BCE_0595) CSB-YP352860BQN
CSB-EP352860BQN
CSB-BP352860BQN
CSB-MP352860BQN
CSB-EP352860BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transcriptional regulatory protein DIP1378 (DIP1378) CSB-YP352861CQF
CSB-EP352861CQF
CSB-BP352861CQF
CSB-MP352861CQF
CSB-EP352861CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Probable transcriptional regulatory protein TT_C0469 (TT_C0469) CSB-YP352862TNS
CSB-EP352862TNS
CSB-BP352862TNS
CSB-MP352862TNS
CSB-EP352862TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus L-lactate dehydrogenase 1 (ldh1) CSB-YP352863BQN
CSB-EP352863BQN
CSB-BP352863BQN
CSB-MP352863BQN
CSB-EP352863BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii L-lactate dehydrogenase 2 (ldh2) CSB-YP352864LAW
CSB-EP352864LAW
CSB-BP352864LAW
CSB-MP352864LAW
CSB-EP352864LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate N-acetyltransferase (argJ) CSB-YP352865CQF
CSB-EP352865CQF
CSB-BP352865CQF
CSB-MP352865CQF
CSB-EP352865CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP352866MLA
CSB-EP352866MLA
CSB-BP352866MLA
CSB-MP352866MLA
CSB-EP352866MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized HTH-type transcriptional regulator SA2060 (SA2060) CSB-YP352867SKY
CSB-EP352867SKY
CSB-BP352867SKY
CSB-MP352867SKY
CSB-EP352867SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem I iron-sulfur center (psaC) CSB-YP352868DOA
CSB-EP352868DOA
CSB-BP352868DOA
CSB-MP352868DOA
CSB-EP352868DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem II reaction center protein I (psbI), partial CSB-YP352869DOA
CSB-EP352869DOA
CSB-BP352869DOA
CSB-MP352869DOA
CSB-EP352869DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein I (psbI), partial CSB-YP352870LQQ
CSB-EP352870LQQ
CSB-BP352870LQQ
CSB-MP352870LQQ
CSB-EP352870LQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem II reaction center protein M (psbM), partial CSB-YP352871DOA
CSB-EP352871DOA
CSB-BP352871DOA
CSB-MP352871DOA
CSB-EP352871DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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