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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bovine Histidine triad nucleotide-binding protein 1 (HINT1) CSB-YP352385BO
CSB-EP352385BO
CSB-BP352385BO
CSB-MP352385BO
CSB-EP352385BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Polyubiquitin CSB-YP352386XBE
CSB-EP352386XBE
CSB-BP352386XBE
CSB-MP352386XBE
CSB-EP352386XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides noxius Alpha-toxin Cn12 CSB-YP352387DRB
CSB-EP352387DRB
CSB-BP352387DRB
CSB-MP352387DRB
CSB-EP352387DRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fe/S biogenesis protein NfuA (nfuA) CSB-YP352388SZB
CSB-EP352388SZB
CSB-BP352388SZB
CSB-MP352388SZB
CSB-EP352388SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Guanine nucleotide-binding protein G (s) subunit alpha isoforms short CSB-YP352389HU
CSB-BP352389HU
CSB-MP352389HU
CSB-EP352389HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HERV-K_1q23.3 provirus ancestral Pro protein CSB-YP352390HU
CSB-EP352390HU
CSB-BP352390HU
CSB-MP352390HU
CSB-EP352390HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HERV-K_6q14.1 provirus ancestral Pro protein CSB-YP352391HU
CSB-EP352391HU
CSB-BP352391HU
CSB-MP352391HU
CSB-EP352391HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Endogenous retrovirus group K member 8 Pol protein (ERVK-8), partial CSB-YP352392HU
CSB-EP352392HU
CSB-BP352392HU
CSB-MP352392HU
CSB-EP352392HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus viridis viridis Myotoxin-2 CSB-YP352393DYG
CSB-EP352393DYG
CSB-BP352393DYG
CSB-MP352393DYG
CSB-EP352393DYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein grpE (grpE) CSB-YP352396AYS
CSB-EP352396AYS
CSB-BP352396AYS
CSB-MP352396AYS
CSB-EP352396AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae 30S ribosomal protein S12 (rpsL) CSB-YP352397HTA
CSB-EP352397HTA
CSB-BP352397HTA
CSB-MP352397HTA
CSB-EP352397HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator gadW (gadW) CSB-YP352398ENV
CSB-EP352398ENV
CSB-BP352398ENV
CSB-MP352398ENV
CSB-EP352398ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulator gadE (gadE) CSB-YP352399EGX
CSB-EP352399EGX
CSB-BP352399EGX
CSB-MP352399EGX
CSB-EP352399EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Phosphoheptose isomerase (gmhA) CSB-YP352400SXB
CSB-EP352400SXB
CSB-BP352400SXB
CSB-MP352400SXB
CSB-EP352400SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoheptose isomerase (gmhA) CSB-YP352401ENV
CSB-BP352401ENV
CSB-MP352401ENV
CSB-EP352401ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D,D-heptose 1,7-bisphosphate phosphatase (gmhB) CSB-YP352402ENV
CSB-EP352402ENV
CSB-BP352402ENV
CSB-MP352402ENV
CSB-EP352402ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP352403IFY1
CSB-EP352403IFY1
CSB-BP352403IFY1
CSB-MP352403IFY1
CSB-EP352403IFY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP352404IFY
CSB-EP352404IFY
CSB-BP352404IFY
CSB-MP352404IFY
CSB-EP352404IFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Cold shock-like protein CspE (cspE) CSB-YP352405BWZ
CSB-EP352405BWZ
CSB-BP352405BWZ
CSB-MP352405BWZ
CSB-EP352405BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Curved DNA-binding protein (cbpA) CSB-YP352406SWW
CSB-EP352406SWW
CSB-BP352406SWW
CSB-MP352406SWW
CSB-EP352406SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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