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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shikimate dehydrogenase (aroE) CSB-YP352467FMW
CSB-EP352467FMW
CSB-BP352467FMW
CSB-MP352467FMW
CSB-EP352467FMW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Shikimate kinase 2 (aroL) CSB-YP352468SXB
CSB-EP352468SXB
CSB-BP352468SXB
CSB-MP352468SXB
CSB-EP352468SXB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chorismate synthase (aroC) CSB-YP352469EGX
CSB-EP352469EGX
CSB-BP352469EGX
CSB-MP352469EGX
CSB-EP352469EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chorismate synthase (aroC) CSB-YP352470SKX
CSB-EP352470SKX
CSB-BP352470SKX
CSB-MP352470SKX
CSB-EP352470SKX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-dehydroquinate synthase (aroB) CSB-YP352471SKX
CSB-EP352471SKX
CSB-BP352471SKX
CSB-MP352471SKX
CSB-EP352471SKX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Putative arsenate reductase (arsC) CSB-YP352472NGG
CSB-EP352472NGG
CSB-BP352472NGG
CSB-MP352472NGG
CSB-EP352472NGG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine dehydrogenase (hom) CSB-YP352473MVZ
CSB-EP352473MVZ
CSB-BP352473MVZ
CSB-MP352473MVZ
CSB-EP352473MVZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Glutamate racemase (murI) CSB-YP352474BMQ
CSB-EP352474BMQ
CSB-BP352474BMQ
CSB-MP352474BMQ
CSB-EP352474BMQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP352475MVH
CSB-EP352475MVH
CSB-BP352475MVH
CSB-MP352475MVH
CSB-EP352475MVH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate racemase (murI) CSB-YP352476SUL
CSB-EP352476SUL
CSB-BP352476SUL
CSB-MP352476SUL
CSB-EP352476SUL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Putative arginyl-tRNA--protein transferase (ate) CSB-YP352477BMO
CSB-EP352477BMO
CSB-BP352477BMO
CSB-MP352477BMO
CSB-EP352477BMO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (scoA) CSB-YP352478MVZ
CSB-EP352478MVZ
CSB-BP352478MVZ
CSB-MP352478MVZ
CSB-EP352478MVZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (scoB) CSB-YP352479MVH
CSB-EP352479MVH
CSB-BP352479MVH
CSB-MP352479MVH
CSB-EP352479MVH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 ATP synthase epsilon chain (atpC) CSB-YP352480BMO
CSB-EP352480BMO
CSB-BP352480BMO
CSB-MP352480BMO
CSB-EP352480BMO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP352481MVH
CSB-EP352481MVH
CSB-BP352481MVH
CSB-MP352481MVH
CSB-EP352481MVH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP352482FMW
CSB-EP352482FMW
CSB-BP352482FMW
CSB-MP352482FMW
CSB-EP352482FMW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potassium-transporting ATPase B chain (kdpB), partial CSB-YP352483MVH1
CSB-EP352483MVH1
CSB-BP352483MVH1
CSB-MP352483MVH1
CSB-EP352483MVH1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting P-type ATPase D (ctpD), partial CSB-YP352484MVH1
CSB-EP352484MVH1
CSB-BP352484MVH1
CSB-MP352484MVH1
CSB-EP352484MVH1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting ATPase G (ctpG), partial CSB-YP352485MVZ
CSB-EP352485MVZ
CSB-BP352485MVZ
CSB-MP352485MVZ
CSB-EP352485MVZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv1488/MT1533.2 (Rv1488, MT1533.2), partial CSB-YP352486MVZ
CSB-EP352486MVZ
CSB-BP352486MVZ
CSB-MP352486MVZ
CSB-EP352486MVZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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