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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella melitensis biotype 1 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP353898BMO
CSB-EP353898BMO
CSB-BP353898BMO
CSB-MP353898BMO
CSB-EP353898BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipase 1 (lip1) CSB-YP353899SKY
CSB-EP353899SKY
CSB-BP353899SKY
CSB-MP353899SKY
CSB-EP353899SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized lipoprotein ygdI (ygdI) CSB-YP353900ENV
CSB-EP353900ENV
CSB-BP353900ENV
CSB-MP353900ENV
CSB-EP353900ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein lppK (lppK) CSB-YP353901MVH
CSB-EP353901MVH
CSB-BP353901MVH
CSB-MP353901MVH
CSB-EP353901MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein lppX (lppX) CSB-YP353902MVH
CSB-EP353902MVH
CSB-BP353902MVH
CSB-MP353902MVH
CSB-EP353902MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein lpqV (lpqV) CSB-YP353903MVZ
CSB-EP353903MVZ
CSB-BP353903MVZ
CSB-MP353903MVZ
CSB-EP353903MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein lprE (lprE) CSB-YP353904MVH
CSB-EP353904MVH
CSB-BP353904MVH
CSB-MP353904MVH
CSB-EP353904MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP353905BMQ
CSB-EP353905BMQ
CSB-BP353905BMQ
CSB-MP353905BMQ
CSB-EP353905BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-ribosylhomocysteine lyase (luxS) CSB-YP353907SKX
CSB-EP353907SKX
CSB-BP353907SKX
CSB-MP353907SKX
CSB-EP353907SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-alpha-glucanotransferase (malQ), partial CSB-YP353908MVH
CSB-EP353908MVH
CSB-BP353908MVH
CSB-MP353908MVH
CSB-EP353908MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized methyltransferase Rv0089/MT0098 (Rv0089, MT0098) CSB-YP353909MVZ
CSB-EP353909MVZ
CSB-BP353909MVZ
CSB-MP353909MVZ
CSB-EP353909MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized methyltransferase Mb3374 (Mb3374) CSB-YP353910MVH
CSB-EP353910MVH
CSB-BP353910MVH
CSB-MP353910MVH
CSB-EP353910MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Septum site-determining protein MinC (minC) CSB-YP353911SWW
CSB-EP353911SWW
CSB-BP353911SWW
CSB-MP353911SWW
CSB-EP353911SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cell division topological specificity factor (minE) CSB-YP353912SXB
CSB-EP353912SXB
CSB-BP353912SXB
CSB-MP353912SXB
CSB-EP353912SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yqcA (yqcA) CSB-YP353913EOD
CSB-EP353913EOD
CSB-BP353913EOD
CSB-MP353913EOD
CSB-EP353913EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein mmpL10 (mmpL10), partial CSB-YP353914MVH
CSB-EP353914MVH
CSB-BP353914MVH
CSB-MP353914MVH
CSB-EP353914MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein mmpS3 (mmpS3), partial CSB-YP353915MVZ1
CSB-EP353915MVZ1
CSB-BP353915MVZ1
CSB-MP353915MVZ1
CSB-EP353915MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP353916EGX
CSB-EP353916EGX
CSB-BP353916EGX
CSB-MP353916EGX
CSB-EP353916EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein A 2 (moaA2) CSB-YP353917MVZ
CSB-EP353917MVZ
CSB-BP353917MVZ
CSB-MP353917MVZ
CSB-EP353917MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP353918SKY
CSB-EP353918SKY
CSB-BP353918SKY
CSB-MP353918SKY
CSB-EP353918SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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