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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Uncharacterized protein yobF (yobF) CSB-YP354910SZB
CSB-EP354910SZB
CSB-BP354910SZB
CSB-MP354910SZB
CSB-EP354910SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein mgrB (mgrB), partial CSB-YP354911SZB
CSB-EP354911SZB
CSB-BP354911SZB
CSB-MP354911SZB
CSB-EP354911SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yodC (yodC) CSB-YP354912ENV
CSB-EP354912ENV
CSB-BP354912ENV
CSB-MP354912ENV
CSB-EP354912ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yeeT (yeeT) CSB-YP354913ENV
CSB-EP354913ENV
CSB-BP354913ENV
CSB-MP354913ENV
CSB-EP354913ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin CbtA (cbtA) CSB-YP354914EGX
CSB-EP354914EGX
CSB-BP354914EGX
CSB-MP354914EGX
CSB-EP354914EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yeeW (yeeW) CSB-YP354915EOD
CSB-EP354915EOD
CSB-BP354915EOD
CSB-MP354915EOD
CSB-EP354915EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin YoeB (yoeB) CSB-YP354916MVH
CSB-EP354916MVH
CSB-BP354916MVH
CSB-MP354916MVH
CSB-EP354916MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nickel/cobalt homeostasis protein RcnB (rcnB) CSB-YP354917ENV
CSB-EP354917ENV
CSB-BP354917ENV
CSB-MP354917ENV
CSB-EP354917ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yeiS (yeiS), partial CSB-YP354918EOD1
CSB-EP354918EOD1
CSB-BP354918EOD1
CSB-MP354918EOD1
CSB-EP354918EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yfcL (yfcL) CSB-YP354919EOD
CSB-EP354919EOD
CSB-BP354919EOD
CSB-MP354919EOD
CSB-EP354919EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yfgG (yfgG), partial CSB-YP354920EOD1
CSB-EP354920EOD1
CSB-BP354920EOD1
CSB-MP354920EOD1
CSB-EP354920EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-alanine exporter AlaE (alaE), partial CSB-YP354921EGX1
CSB-EP354921EGX1
CSB-BP354921EGX1
CSB-MP354921EGX1
CSB-EP354921EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 7-carboxy-7-deazaguanine synthase (queE) CSB-YP354922ENV
CSB-EP354922ENV
CSB-BP354922ENV
CSB-MP354922ENV
CSB-EP354922ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 7-carboxy-7-deazaguanine synthase (queE) CSB-YP354923SZB
CSB-EP354923SZB
CSB-BP354923SZB
CSB-MP354923SZB
CSB-EP354923SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0350 protein ygfY (ygfY) CSB-YP354924ENV
CSB-EP354924ENV
CSB-BP354924ENV
CSB-MP354924ENV
CSB-EP354924ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yqfE (yqfE) CSB-YP354925EGX
CSB-EP354925EGX
CSB-BP354925EGX
CSB-MP354925EGX
CSB-EP354925EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yqgB (yqgB) CSB-YP354926ENV
CSB-EP354926ENV
CSB-BP354926ENV
CSB-MP354926ENV
CSB-EP354926ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yqgC (yqgC) CSB-YP354927EOD
CSB-EP354927EOD
CSB-BP354927EOD
CSB-MP354927EOD
CSB-EP354927EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yghW (yghW) CSB-YP354928EOD
CSB-EP354928EOD
CSB-BP354928EOD
CSB-MP354928EOD
CSB-EP354928EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yqjD (yqjD), partial CSB-YP354929EGX1
CSB-EP354929EGX1
CSB-BP354929EGX1
CSB-MP354929EGX1
CSB-EP354929EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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