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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP355395SKX
CSB-EP355395SKX
CSB-BP355395SKX
CSB-MP355395SKX
CSB-EP355395SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP355396MVZ
CSB-EP355396MVZ
CSB-BP355396MVZ
CSB-MP355396MVZ
CSB-EP355396MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP355397SKY
CSB-EP355397SKY
CSB-BP355397SKY
CSB-MP355397SKY
CSB-EP355397SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP355398BMO
CSB-EP355398BMO
CSB-BP355398BMO
CSB-MP355398BMO
CSB-EP355398BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP355399SXB
CSB-EP355399SXB
CSB-BP355399SXB
CSB-MP355399SXB
CSB-EP355399SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP355400SKY
CSB-EP355400SKY
CSB-BP355400SKY
CSB-MP355400SKY
CSB-EP355400SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP355401SKX
CSB-EP355401SKX
CSB-BP355401SKX
CSB-MP355401SKX
CSB-EP355401SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP355402MVZ
CSB-EP355402MVZ
CSB-BP355402MVZ
CSB-MP355402MVZ
CSB-EP355402MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium S-adenosylmethionine synthase (metK) CSB-YP355403SXB
CSB-EP355403SXB
CSB-BP355403SXB
CSB-MP355403SXB
CSB-EP355403SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein SPy_0538/M5005_Spy0445 (metK1) CSB-YP355404SMT
CSB-EP355404SMT
CSB-BP355404SMT
CSB-MP355404SMT
CSB-EP355404SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-serine dehydratase (sdaA) CSB-YP355405MVH
CSB-EP355405MVH
CSB-BP355405MVH
CSB-MP355405MVH
CSB-EP355405MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase Rv1543/MT1595 (Rv1543, MT1595) CSB-YP355406MVZ
CSB-EP355406MVZ
CSB-BP355406MVZ
CSB-MP355406MVZ
CSB-EP355406MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein translocase subunit SecA 2 (secA2), partial CSB-YP355407MVH
CSB-EP355407MVH
CSB-BP355407MVH
CSB-MP355407MVH
CSB-EP355407MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Selenide, water dikinase (selD) CSB-YP355408EOD
CSB-EP355408EOD
CSB-BP355408EOD
CSB-MP355408EOD
CSB-EP355408EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Response regulator uvrY (uvrY) CSB-YP355409EOD
CSB-EP355409EOD
CSB-BP355409EOD
CSB-MP355409EOD
CSB-EP355409EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-E factor (rpoE) CSB-YP355410MVH
CSB-EP355410MVH
CSB-BP355410MVH
CSB-MP355410MVH
CSB-EP355410MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable RNA polymerase sigma-D factor (sigD) CSB-YP355411MVZ
CSB-EP355411MVZ
CSB-BP355411MVZ
CSB-MP355411MVZ
CSB-EP355411MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD-dependent protein deacylase (cobB) CSB-YP355412MVZ
CSB-EP355412MVZ
CSB-BP355412MVZ
CSB-MP355412MVZ
CSB-EP355412MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NAD-dependent protein deacetylase (cobB) CSB-YP355413SKY
CSB-EP355413SKY
CSB-BP355413SKY
CSB-MP355413SKY
CSB-EP355413SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Protein smg homolog (smg) CSB-YP355414NGF
CSB-EP355414NGF
CSB-BP355414NGF
CSB-MP355414NGF
CSB-EP355414NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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