重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Staphylococcus aureus Probable DNA-directed RNA polymerase subunit delta (rpoE) | CSB-YP355395SKX CSB-EP355395SKX CSB-BP355395SKX CSB-MP355395SKX CSB-EP355395SKX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant DNA-directed RNA polymerase subunit omega (rpoZ) | CSB-YP355396MVZ CSB-EP355396MVZ CSB-BP355396MVZ CSB-MP355396MVZ CSB-EP355396MVZ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Staphylococcus aureus DNA-directed RNA polymerase subunit omega (rpoZ) | CSB-YP355397SKY CSB-EP355397SKY CSB-BP355397SKY CSB-MP355397SKY CSB-EP355397SKY-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Brucella melitensis biotype 1 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) | CSB-YP355398BMO CSB-EP355398BMO CSB-BP355398BMO CSB-MP355398BMO CSB-EP355398BMO-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Salmonella typhimurium Holliday junction ATP-dependent DNA helicase RuvA (ruvA) | CSB-YP355399SXB CSB-EP355399SXB CSB-BP355399SXB CSB-MP355399SXB CSB-EP355399SXB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) | CSB-YP355400SKY CSB-EP355400SKY CSB-BP355400SKY CSB-MP355400SKY CSB-EP355400SKY-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) | CSB-YP355401SKX CSB-EP355401SKX CSB-BP355401SKX CSB-MP355401SKX CSB-EP355401SKX-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Crossover junction endodeoxyribonuclease RuvC (ruvC) | CSB-YP355402MVZ CSB-EP355402MVZ CSB-BP355402MVZ CSB-MP355402MVZ CSB-EP355402MVZ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Salmonella typhimurium S-adenosylmethionine synthase (metK) | CSB-YP355403SXB CSB-EP355403SXB CSB-BP355403SXB CSB-MP355403SXB CSB-EP355403SXB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Uncharacterized protein SPy_0538/M5005_Spy0445 (metK1) | CSB-YP355404SMT CSB-EP355404SMT CSB-BP355404SMT CSB-MP355404SMT CSB-EP355404SMT-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant L-serine dehydratase (sdaA) | CSB-YP355405MVH CSB-EP355405MVH CSB-BP355405MVH CSB-MP355405MVH CSB-EP355405MVH-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Uncharacterized oxidoreductase Rv1543/MT1595 (Rv1543, MT1595) | CSB-YP355406MVZ CSB-EP355406MVZ CSB-BP355406MVZ CSB-MP355406MVZ CSB-EP355406MVZ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Protein translocase subunit SecA 2 (secA2), partial | CSB-YP355407MVH CSB-EP355407MVH CSB-BP355407MVH CSB-MP355407MVH CSB-EP355407MVH-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Selenide, water dikinase (selD) | CSB-YP355408EOD CSB-EP355408EOD CSB-BP355408EOD CSB-MP355408EOD CSB-EP355408EOD-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Response regulator uvrY (uvrY) | CSB-YP355409EOD CSB-EP355409EOD CSB-BP355409EOD CSB-MP355409EOD CSB-EP355409EOD-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant RNA polymerase sigma-E factor (rpoE) | CSB-YP355410MVH CSB-EP355410MVH CSB-BP355410MVH CSB-MP355410MVH CSB-EP355410MVH-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Probable RNA polymerase sigma-D factor (sigD) | CSB-YP355411MVZ CSB-EP355411MVZ CSB-BP355411MVZ CSB-MP355411MVZ CSB-EP355411MVZ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant NAD-dependent protein deacylase (cobB) | CSB-YP355412MVZ CSB-EP355412MVZ CSB-BP355412MVZ CSB-MP355412MVZ CSB-EP355412MVZ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Staphylococcus aureus NAD-dependent protein deacetylase (cobB) | CSB-YP355413SKY CSB-EP355413SKY CSB-BP355413SKY CSB-MP355413SKY CSB-EP355413SKY-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Neisseria meningitidis serogroup A / serotype 4A Protein smg homolog (smg) | CSB-YP355414NGF CSB-EP355414NGF CSB-BP355414NGF CSB-MP355414NGF CSB-EP355414NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022