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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UPF0060 membrane protein Rv2639c/MT2717 (Rv2639c, MT2717), partial CSB-YP355478MVZ1
CSB-EP355478MVZ1
CSB-BP355478MVZ1
CSB-MP355478MVZ1
CSB-EP355478MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0060 membrane protein MW2259 (MW2259), partial CSB-YP355479SUL1
CSB-EP355479SUL1
CSB-BP355479SUL1
CSB-MP355479SUL1
CSB-EP355479SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0073 inner membrane protein yqfA (yqfA), partial CSB-YP355480EGX1
CSB-EP355480EGX1
CSB-BP355480EGX1
CSB-MP355480EGX1
CSB-EP355480EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0073 membrane protein Mb1114c (Mb1114c), partial CSB-YP355481MVH1
CSB-EP355481MVH1
CSB-BP355481MVH1
CSB-MP355481MVH1
CSB-EP355481MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP355482SKX1
CSB-EP355482SKX1
CSB-BP355482SKX1
CSB-MP355482SKX1
CSB-EP355482SKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP355483SMI1
CSB-EP355483SMI1
CSB-BP355483SMI1
CSB-MP355483SMI1
CSB-EP355483SMI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Steroid 21-hydroxylase (CYP21) CSB-YP355484BO
CSB-EP355484BO
CSB-BP355484BO
CSB-MP355484BO
CSB-EP355484BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacterium thermosaccharolyticum Ferredoxin CSB-YP355485TDA
CSB-EP355485TDA
CSB-BP355485TDA
CSB-MP355485TDA
CSB-EP355485TDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP355486CJL
CSB-EP355486CJL
CSB-BP355486CJL
CSB-MP355486CJL
CSB-EP355486CJL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-2 CSB-YP355487DJO
CSB-EP355487DJO
CSB-BP355487DJO
CSB-MP355487DJO
CSB-EP355487DJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-1 (fdxA) CSB-YP355488DPX
CSB-EP355488DPX
CSB-BP355488DPX
CSB-MP355488DPX
CSB-EP355488DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Ferredoxin (fdx) CSB-YP355489HTM
CSB-EP355489HTM
CSB-BP355489HTM
CSB-MP355489HTM
CSB-EP355489HTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Ferredoxin-1, chloroplastic (PETF) CSB-YP355490FKI
CSB-EP355490FKI
CSB-BP355490FKI
CSB-MP355490FKI
CSB-EP355490FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Ferredoxin CSB-YP355491BWD
CSB-EP355491BWD
CSB-BP355491BWD
CSB-MP355491BWD
CSB-EP355491BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytolacca acinosa Ferredoxin-1 CSB-YP355492PJH
CSB-EP355492PJH
CSB-BP355492PJH
CSB-MP355492PJH
CSB-EP355492PJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equisetum telmateia Ferredoxin-1 CSB-YP355493EGA
CSB-EP355493EGA
CSB-BP355493EGA
CSB-MP355493EGA
CSB-EP355493EGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP355494SDJ
CSB-EP355494SDJ
CSB-BP355494SDJ
CSB-MP355494SDJ
CSB-EP355494SDJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin (PETF) CSB-YP355495DZV
CSB-EP355495DZV
CSB-BP355495DZV
CSB-MP355495DZV
CSB-EP355495DZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-1 CSB-YP355496AMA
CSB-EP355496AMA
CSB-BP355496AMA
CSB-MP355496AMA
CSB-EP355496AMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP355497CJP
CSB-EP355497CJP
CSB-BP355497CJP
CSB-MP355497CJP
CSB-EP355497CJP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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