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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup B 30S ribosomal protein S18 (rpsR) CSB-YP357975NGG
CSB-EP357975NGG
CSB-BP357975NGG
CSB-MP357975NGG
CSB-EP357975NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 30S ribosomal protein S4 (rpsD) CSB-YP357976XAY
CSB-EP357976XAY
CSB-BP357976XAY
CSB-MP357976XAY
CSB-EP357976XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP357977XAY
CSB-EP357977XAY
CSB-BP357977XAY
CSB-MP357977XAY
CSB-EP357977XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Major ferric iron-binding protein (fbpA) CSB-YP357978NGF
CSB-EP357978NGF
CSB-BP357978NGF
CSB-MP357978NGF
CSB-EP357978NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Phosphoheptose isomerase (gmhA) CSB-YP357979NGF
CSB-EP357979NGF
CSB-BP357979NGF
CSB-MP357979NGF
CSB-EP357979NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A SsrA-binding protein (smpB) CSB-YP357980NGF
CSB-EP357980NGF
CSB-BP357980NGF
CSB-MP357980NGF
CSB-EP357980NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Putative HTH-type transcriptional regulator NMA1593 (NMA1593) CSB-YP357981NGF
CSB-EP357981NGF
CSB-BP357981NGF
CSB-MP357981NGF
CSB-EP357981NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0271 protein rni3 (rni3) CSB-YP357982NGF
CSB-EP357982NGF
CSB-BP357982NGF
CSB-MP357982NGF
CSB-EP357982NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP357983NGF
CSB-EP357983NGF
CSB-BP357983NGF
CSB-MP357983NGF
CSB-EP357983NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Penicillin-binding protein 1A (mrcA), partial CSB-YP357984NGF
CSB-EP357984NGF
CSB-BP357984NGF
CSB-MP357984NGF
CSB-EP357984NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Amino-acid acetyltransferase (argA) CSB-YP357985FGB
CSB-EP357985FGB
CSB-BP357985FGB
CSB-MP357985FGB
CSB-EP357985FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-oxoadipate CoA-transferase subunit B (pcaJ) CSB-YP357986FGB
CSB-EP357986FGB
CSB-BP357986FGB
CSB-MP357986FGB
CSB-EP357986FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP synthase protein I (atpI), partial CSB-YP357987FGB1
CSB-EP357987FGB1
CSB-BP357987FGB1
CSB-MP357987FGB1
CSB-EP357987FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Cold shock protein CapB (capB) CSB-YP357988FGP
CSB-EP357988FGP
CSB-BP357988FGP
CSB-MP357988FGP
CSB-EP357988FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,2-dihydroxynaphthalene dioxygenase (nahC) CSB-YP357989EZW
CSB-EP357989EZW
CSB-BP357989EZW
CSB-MP357989EZW
CSB-EP357989EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 1,2-dihydroxynaphthalene dioxygenase (nahC) CSB-YP357990FFZ
CSB-EP357990FFZ
CSB-BP357990FFZ
CSB-MP357990FFZ
CSB-EP357990FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Naphthalene 1,2-dioxygenase subunit alpha (doxB) CSB-YP357991PVJ
CSB-EP357991PVJ
CSB-BP357991PVJ
CSB-MP357991PVJ
CSB-EP357991PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Naphthalene 1,2-dioxygenase subunit beta (doxD) CSB-YP357992PVJ
CSB-EP357992PVJ
CSB-BP357992PVJ
CSB-MP357992PVJ
CSB-EP357992PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Carboxymethylenebutenolidase (clcD) CSB-YP357993FGG
CSB-EP357993FGG
CSB-BP357993FGG
CSB-MP357993FGG
CSB-EP357993FGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP357994FFZ
CSB-EP357994FFZ
CSB-BP357994FFZ
CSB-MP357994FFZ
CSB-EP357994FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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