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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida DNA primase (dnaG), partial CSB-YP357995FGB
CSB-EP357995FGB
CSB-BP357995FGB
CSB-MP357995FGB
CSB-EP357995FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA polymerase III subunit beta (dnaN) CSB-YP357996FFZ
CSB-EP357996FFZ
CSB-BP357996FFZ
CSB-MP357996FFZ
CSB-EP357996FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP357997FGB
CSB-EP357997FGB
CSB-BP357997FGB
CSB-MP357997FGB
CSB-EP357997FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Integration host factor subunit alpha (ihfA) CSB-YP357998FFZ
CSB-EP357998FFZ
CSB-BP357998FFZ
CSB-MP357998FFZ
CSB-EP357998FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Integration host factor subunit beta (ihfB) CSB-YP357999FGB
CSB-EP357999FGB
CSB-BP357999FGB
CSB-MP357999FGB
CSB-EP357999FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequences IS1326/IS1353 (istA), partial CSB-YP358001EZW
CSB-EP358001EZW
CSB-BP358001EZW
CSB-MP358001EZW
CSB-EP358001EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Adenylate kinase (adk) CSB-YP358002FGB
CSB-EP358002FGB
CSB-BP358002FGB
CSB-MP358002FGB
CSB-EP358002FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Peptidoglycan-associated lipoprotein (pal) CSB-YP358003FFZ
CSB-EP358003FFZ
CSB-BP358003FFZ
CSB-MP358003FFZ
CSB-EP358003FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Membrane protein insertase YidC (yidC), partial CSB-YP358004FFZ1
CSB-EP358004FFZ1
CSB-BP358004FFZ1
CSB-MP358004FFZ1
CSB-EP358004FFZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trans-O-hydroxybenzylidenepyruvate hydratase-aldolase (pahE) CSB-YP358005EZW
CSB-EP358005EZW
CSB-BP358005EZW
CSB-MP358005EZW
CSB-EP358005EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dibenzothiophene metabolism operon protein pahQ (pahQ) CSB-YP358006EZW
CSB-EP358006EZW
CSB-BP358006EZW
CSB-MP358006EZW
CSB-EP358006EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Dibenzothiophene metabolism operon protein doxH (doxH) CSB-YP358007PVJ
CSB-EP358007PVJ
CSB-BP358007PVJ
CSB-MP358007PVJ
CSB-EP358007PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosome hibernation promoting factor (hpf) CSB-YP358008FFZ
CSB-EP358008FFZ
CSB-BP358008FFZ
CSB-MP358008FFZ
CSB-EP358008FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable chromosome-partitioning protein parB (parB) CSB-YP358010FFZ
CSB-EP358010FFZ
CSB-BP358010FFZ
CSB-MP358010FFZ
CSB-EP358010FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida LexA repressor (lexA) CSB-YP358011FFZ
CSB-EP358011FFZ
CSB-BP358011FFZ
CSB-MP358011FFZ
CSB-EP358011FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alkyl hydroperoxide reductase subunit F (ahpF), partial CSB-YP358012FFZ
CSB-EP358012FFZ
CSB-BP358012FFZ
CSB-MP358012FFZ
CSB-EP358012FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L7/L12 (rplL) CSB-YP358013FFZ
CSB-EP358013FFZ
CSB-BP358013FFZ
CSB-MP358013FFZ
CSB-EP358013FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP358014PWD
CSB-EP358014PWD
CSB-BP358014PWD
CSB-MP358014PWD
CSB-EP358014PWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L34 (rpmH) CSB-YP358015FFZ
CSB-EP358015FFZ
CSB-BP358015FFZ
CSB-MP358015FFZ
CSB-EP358015FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP358016PWD
CSB-EP358016PWD
CSB-BP358016PWD
CSB-MP358016PWD
CSB-EP358016PWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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