Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechococcus elongatus Protein translocase subunit SecY (secY), partial CSB-YP358526FPY
CSB-EP358526FPY
CSB-BP358526FPY
CSB-MP358526FPY
CSB-EP358526FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Virulence factors putative positive transcription regulator BvgA (bvgA) CSB-YP358527BUA
CSB-EP358527BUA
CSB-BP358527BUA
CSB-MP358527BUA
CSB-EP358527BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Virulence factors putative positive transcription regulator BvgA (bvgA) CSB-YP358528BIK
CSB-EP358528BIK
CSB-BP358528BIK
CSB-MP358528BIK
CSB-EP358528BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Chemotaxis protein CheY (cheY) CSB-YP358529LGU
CSB-EP358529LGU
CSB-BP358529LGU
CSB-MP358529LGU
CSB-EP358529LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Transcriptional regulatory protein CiaR (ciaR) CSB-YP358530SMP
CSB-EP358530SMP
CSB-BP358530SMP
CSB-MP358530SMP
CSB-EP358530SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulatory protein dltR (dltR) CSB-YP358531SMI
CSB-EP358531SMI
CSB-BP358531SMI
CSB-MP358531SMI
CSB-EP358531SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulatory protein CutR (cutR) CSB-YP358532FOI
CSB-EP358532FOI
CSB-BP358532FOI
CSB-MP358532FOI
CSB-EP358532FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stage 0 sporulation protein A (spo0A) CSB-YP358533BRN
CSB-EP358533BRN
CSB-BP358533BRN
CSB-MP358533BRN
CSB-EP358533BRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Sensor protein CiaH (ciaH), partial CSB-YP358534SMP1
CSB-EP358534SMP1
CSB-BP358534SMP1
CSB-MP358534SMP1
CSB-EP358534SMP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sensor protein CutS (cutS), partial CSB-YP358535FOI1
CSB-EP358535FOI1
CSB-BP358535FOI1
CSB-MP358535FOI1
CSB-EP358535FOI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae RNA polymerase sigma factor rpoD (rpoD) CSB-YP358536SMP
CSB-EP358536SMP
CSB-BP358536SMP
CSB-MP358536SMP
CSB-EP358536SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn/Fe] (sodA) CSB-YP358537SMI
CSB-EP358537SMI
CSB-BP358537SMI
CSB-MP358537SMI
CSB-EP358537SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Anti-sigma-B factor antagonist (rsbV) CSB-YP358538LPY
CSB-EP358538LPY
CSB-BP358538LPY
CSB-MP358538LPY
CSB-EP358538LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Single-stranded DNA-binding protein 1 (ssb1) CSB-YP358539NHR
CSB-EP358539NHR
CSB-BP358539NHR
CSB-MP358539NHR
CSB-EP358539NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Cystathionine beta-lyase (metC) CSB-YP358540LNG
CSB-EP358540LNG
CSB-BP358540LNG
CSB-MP358540LNG
CSB-EP358540LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Multi-drug resistance efflux pump pmrA (pmrA), partial CSB-YP358541SMP
CSB-EP358541SMP
CSB-BP358541SMP
CSB-MP358541SMP
CSB-EP358541SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracycline resistance protein (tet), partial CSB-YP358542BQJ
CSB-EP358542BQJ
CSB-BP358542BQJ
CSB-MP358542BQJ
CSB-EP358542BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Thioredoxin-1 (trxA) CSB-YP358543NHR
CSB-EP358543NHR
CSB-BP358543NHR
CSB-MP358543NHR
CSB-EP358543NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiol:disulfide interchange protein DsbA (dsbA) CSB-YP358544EGX
CSB-EP358544EGX
CSB-BP358544EGX
CSB-MP358544EGX
CSB-EP358544EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiol:disulfide interchange protein DsbA-like (c3786) CSB-YP358545EGX
CSB-EP358545EGX
CSB-BP358545EGX
CSB-MP358545EGX
CSB-EP358545EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>