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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella flexneri Transcription antitermination protein NusB (nusB) CSB-YP358936SZB
CSB-EP358936SZB
CSB-BP358936SZB
CSB-MP358936SZB
CSB-EP358936SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate carbamoyltransferase catalytic subunit (pyrB) CSB-YP358937ENV
CSB-EP358937ENV
CSB-BP358937ENV
CSB-MP358937ENV
CSB-EP358937ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase catalytic chain (pyrB) CSB-YP358938SZB
CSB-EP358938SZB
CSB-BP358938SZB
CSB-MP358938SZB
CSB-EP358938SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate 1-decarboxylase (panD) CSB-YP358939EGX
CSB-EP358939EGX
CSB-BP358939EGX
CSB-MP358939EGX
CSB-EP358939EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate 1-decarboxylase (panD) CSB-YP358940SZB
CSB-EP358940SZB
CSB-BP358940SZB
CSB-MP358940SZB
CSB-EP358940SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6-phosphofructokinase isozyme 1 (pfkA) CSB-YP358942EOD
CSB-EP358942EOD
CSB-BP358942EOD
CSB-MP358942EOD
CSB-EP358942EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoglycerate kinase (pgk) CSB-YP358943ENV
CSB-EP358943ENV
CSB-BP358943ENV
CSB-MP358943ENV
CSB-EP358943ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerate kinase (pgk) CSB-YP358944SZB
CSB-EP358944SZB
CSB-BP358944SZB
CSB-MP358944SZB
CSB-EP358944SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP358945EOD
CSB-EP358945EOD
CSB-BP358945EOD
CSB-MP358945EOD
CSB-EP358945EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP358946ENV
CSB-EP358946ENV
CSB-BP358946ENV
CSB-MP358946ENV
CSB-EP358946ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP358947ENV
CSB-EP358947ENV
CSB-BP358947ENV
CSB-MP358947ENV
CSB-EP358947ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Polyphosphate kinase (ppk) CSB-YP358948ENV
CSB-BP358948ENV
CSB-MP358948ENV
CSB-EP358948ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP358949ENV
CSB-EP358949ENV
CSB-BP358949ENV
CSB-MP358949ENV
CSB-EP358949ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate 5-kinase (proB) CSB-YP358950ENV
CSB-EP358950ENV
CSB-BP358950ENV
CSB-MP358950ENV
CSB-EP358950ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate 5-kinase (proB) CSB-YP358951EOD
CSB-EP358951EOD
CSB-BP358951EOD
CSB-MP358951EOD
CSB-EP358951EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent protease subunit HslV (hslV) CSB-YP358952EGX
CSB-EP358952EGX
CSB-BP358952EGX
CSB-MP358952EGX
CSB-EP358952EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent protease subunit HslV (hslV) CSB-YP358953SZB
CSB-EP358953SZB
CSB-BP358953SZB
CSB-MP358953SZB
CSB-EP358953SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LexA repressor (lexA) CSB-YP358954EGX
CSB-EP358954EGX
CSB-BP358954EGX
CSB-MP358954EGX
CSB-EP358954EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LexA repressor (lexA) CSB-YP358955SZB
CSB-EP358955SZB
CSB-BP358955SZB
CSB-MP358955SZB
CSB-EP358955SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidase E (pepE) CSB-YP358956EOD
CSB-EP358956EOD
CSB-BP358956EOD
CSB-MP358956EOD
CSB-EP358956EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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