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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Chaperone protein hscA (hscA) CSB-YP358896ENV
CSB-EP358896ENV
CSB-BP358896ENV
CSB-MP358896ENV
CSB-EP358896ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein htpG (htpG) CSB-YP358897ENV
CSB-BP358897ENV
CSB-MP358897ENV
CSB-EP358897ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein htpG (htpG) CSB-YP358898EOD
CSB-EP358898EOD
CSB-BP358898EOD
CSB-MP358898EOD
CSB-EP358898EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nickel-responsive regulator (nikR) CSB-YP358899EGX
CSB-EP358899EGX
CSB-BP358899EGX
CSB-MP358899EGX
CSB-EP358899EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nickel-responsive regulator (nikR) CSB-YP358900SZB
CSB-EP358900SZB
CSB-BP358900SZB
CSB-MP358900SZB
CSB-EP358900SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein HypA (hypA) CSB-YP358901EOD
CSB-EP358901EOD
CSB-BP358901EOD
CSB-MP358901EOD
CSB-EP358901EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable hydrogenase nickel incorporation protein HybF (hybF) CSB-YP358902ENV
CSB-EP358902ENV
CSB-BP358902ENV
CSB-MP358902ENV
CSB-EP358902ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable intracellular septation protein A (yciB), partial CSB-YP358903ENV1
CSB-EP358903ENV1
CSB-BP358903ENV1
CSB-MP358903ENV1
CSB-EP358903ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-keto-3-deoxygluconate permease (kdgT), partial CSB-YP358904ENV
CSB-EP358904ENV
CSB-BP358904ENV
CSB-MP358904ENV
CSB-EP358904ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-keto-3-deoxygluconate permease (kdgT), partial CSB-YP358905SZB
CSB-EP358905SZB
CSB-BP358905SZB
CSB-MP358905SZB
CSB-EP358905SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP358906SZB
CSB-EP358906SZB
CSB-BP358906SZB
CSB-MP358906SZB
CSB-EP358906SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribose-phosphate pyrophosphokinase (prs) CSB-YP358907ENV
CSB-EP358907ENV
CSB-BP358907ENV
CSB-MP358907ENV
CSB-EP358907ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase (prs) CSB-YP358908EOD
CSB-EP358908EOD
CSB-BP358908EOD
CSB-MP358908EOD
CSB-EP358908EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP358909EGX
CSB-EP358909EGX
CSB-BP358909EGX
CSB-MP358909EGX
CSB-EP358909EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC) CSB-YP358910ENV
CSB-BP358910ENV
CSB-MP358910ENV
CSB-EP358910ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC) CSB-YP358911EOD
CSB-EP358911EOD
CSB-BP358911EOD
CSB-MP358911EOD
CSB-EP358911EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maf-like protein yceF (yceF) CSB-YP358912ENV
CSB-EP358912ENV
CSB-BP358912ENV
CSB-MP358912ENV
CSB-EP358912ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylglyoxal synthase (mgsA) CSB-YP358913EOD
CSB-EP358913EOD
CSB-BP358913EOD
CSB-MP358913EOD
CSB-EP358913EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cell division topological specificity factor (minE) CSB-YP358914ENV
CSB-EP358914ENV
CSB-BP358914ENV
CSB-MP358914ENV
CSB-EP358914ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division topological specificity factor (minE) CSB-YP358915EOD
CSB-EP358915EOD
CSB-BP358915EOD
CSB-MP358915EOD
CSB-EP358915EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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