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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrogen regulatory protein P-II 2 (glnK) CSB-YP359723EOD
CSB-EP359723EOD
CSB-BP359723EOD
CSB-MP359723EOD
CSB-EP359723EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutaredoxin-like protein nrdH (nrdH) CSB-YP359724ENV
CSB-EP359724ENV
CSB-BP359724ENV
CSB-MP359724ENV
CSB-EP359724ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutaredoxin-like protein nrdH (nrdH) CSB-YP359725EOD
CSB-EP359725EOD
CSB-BP359725EOD
CSB-MP359725EOD
CSB-EP359725EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutaredoxin-4 (grxD) CSB-YP359726ENV
CSB-EP359726ENV
CSB-BP359726ENV
CSB-MP359726ENV
CSB-EP359726ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutaredoxin-4 (grxD) CSB-YP359727EOD
CSB-EP359727EOD
CSB-BP359727EOD
CSB-MP359727EOD
CSB-EP359727EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Evolved beta-galactosidase subunit beta (ebgC) CSB-YP359728ENV
CSB-EP359728ENV
CSB-BP359728ENV
CSB-MP359728ENV
CSB-EP359728ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-deoxy-D-manno-octulosonic-acid transferase (waaA), partial CSB-YP359729ENV
CSB-EP359729ENV
CSB-BP359729ENV
CSB-MP359729ENV
CSB-EP359729ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-deoxy-D-manno-octulosonic-acid transferase (waaA), partial CSB-YP359730EOD
CSB-EP359730EOD
CSB-BP359730EOD
CSB-MP359730EOD
CSB-EP359730EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase (wecA), partial CSB-YP359731EGX
CSB-EP359731EGX
CSB-BP359731EGX
CSB-MP359731EGX
CSB-EP359731EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein gnsA (gnsA) CSB-YP359732ENV
CSB-EP359732ENV
CSB-BP359732ENV
CSB-MP359732ENV
CSB-EP359732ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli High-affinity gluconate transporter (gntP), partial CSB-YP359733ENV
CSB-EP359733ENV
CSB-BP359733ENV
CSB-MP359733ENV
CSB-EP359733ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Low-affinity gluconate transporter (gntU), partial CSB-YP359734ENV
CSB-EP359734ENV
CSB-BP359734ENV
CSB-MP359734ENV
CSB-EP359734ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein yaaH (yaaH), partial CSB-YP359735ENV1
CSB-EP359735ENV1
CSB-BP359735ENV1
CSB-MP359735ENV1
CSB-EP359735ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inner membrane protein yaaH (yaaH), partial CSB-YP359736SZB1
CSB-EP359736SZB1
CSB-BP359736SZB1
CSB-MP359736SZB1
CSB-EP359736SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized GST-like protein yibF (yibF) CSB-YP359737EGX
CSB-EP359737EGX
CSB-BP359737EGX
CSB-MP359737EGX
CSB-EP359737EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stringent starvation protein A (sspA) CSB-YP359738EGX
CSB-EP359738EGX
CSB-BP359738EGX
CSB-MP359738EGX
CSB-EP359738EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stringent starvation protein A (sspA) CSB-YP359739SZB
CSB-EP359739SZB
CSB-BP359739SZB
CSB-MP359739SZB
CSB-EP359739SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutathione S-transferase GstB (gstB) CSB-YP359740EGX
CSB-EP359740EGX
CSB-BP359740EGX
CSB-MP359740EGX
CSB-EP359740EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Replicative DNA helicase (dnaB) CSB-YP359741ENV
CSB-EP359741ENV
CSB-BP359741ENV
CSB-MP359741ENV
CSB-EP359741ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protoporphyrinogen IX dehydrogenase [menaquinone] (hemG) CSB-YP359742ENV
CSB-EP359742ENV
CSB-BP359742ENV
CSB-MP359742ENV
CSB-EP359742ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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