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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Lipoprotein-releasing system transmembrane protein LolC (lolC), partial CSB-YP359923ENV1
CSB-EP359923ENV1
CSB-BP359923ENV1
CSB-MP359923ENV1
CSB-EP359923ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein-releasing system transmembrane protein LolC (lolC), partial CSB-YP359924EOD1
CSB-EP359924EOD1
CSB-BP359924EOD1
CSB-MP359924EOD1
CSB-EP359924EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipopolysaccharide export system permease protein lptG (lptG), partial CSB-YP359925EGX1
CSB-EP359925EGX1
CSB-BP359925EGX1
CSB-MP359925EGX1
CSB-EP359925EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yjiX (yjiX) CSB-YP359926EGX
CSB-EP359926EGX
CSB-BP359926EGX
CSB-MP359926EGX
CSB-EP359926EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0442 protein yjjB (yjjB), partial CSB-YP359927ENV1
CSB-EP359927ENV1
CSB-BP359927ENV1
CSB-MP359927ENV1
CSB-EP359927ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0442 protein yjjB (yjjB), partial CSB-YP359928EOD1
CSB-EP359928EOD1
CSB-BP359928EOD1
CSB-MP359928EOD1
CSB-EP359928EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inner membrane protein yjjP (yjjP), partial CSB-YP359929SZB1
CSB-EP359929SZB1
CSB-BP359929SZB1
CSB-MP359929SZB1
CSB-EP359929SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yjjQ (yjjQ) CSB-YP359930EOD
CSB-EP359930EOD
CSB-BP359930EOD
CSB-MP359930EOD
CSB-EP359930EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yjjY (yjjY) CSB-YP359931EOD
CSB-EP359931EOD
CSB-BP359931EOD
CSB-MP359931EOD
CSB-EP359931EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ytfK (ytfK) CSB-YP359932ENV
CSB-EP359932ENV
CSB-BP359932ENV
CSB-MP359932ENV
CSB-EP359932ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ytfM (ytfM), partial CSB-YP359933ENV
CSB-EP359933ENV
CSB-BP359933ENV
CSB-MP359933ENV
CSB-EP359933ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ygaU (ygaU) CSB-YP359934ENV
CSB-EP359934ENV
CSB-BP359934ENV
CSB-MP359934ENV
CSB-EP359934ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA-modifying protein ygfZ (ygfZ) CSB-YP359935ENV
CSB-EP359935ENV
CSB-BP359935ENV
CSB-MP359935ENV
CSB-EP359935ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative fruR/shl operon leader peptide (fruL) CSB-YP359936ENV
CSB-EP359936ENV
CSB-BP359936ENV
CSB-MP359936ENV
CSB-EP359936ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative fruR/shl operon leader peptide (fruL) CSB-YP359937SZB
CSB-EP359937SZB
CSB-BP359937SZB
CSB-MP359937SZB
CSB-EP359937SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative rho operon leader peptide (rhoL) CSB-YP359938EGX
CSB-EP359938EGX
CSB-BP359938EGX
CSB-MP359938EGX
CSB-EP359938EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphogluconate dehydratase (edd) CSB-YP359939ENV
CSB-EP359939ENV
CSB-BP359939ENV
CSB-MP359939ENV
CSB-EP359939ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetolactate synthase isozyme 1 small subunit (ilvN) CSB-YP359940ENV
CSB-EP359940ENV
CSB-BP359940ENV
CSB-MP359940ENV
CSB-EP359940ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetolactate synthase isozyme 1 small subunit (ilvN) CSB-YP359941EOD
CSB-EP359941EOD
CSB-BP359941EOD
CSB-MP359941EOD
CSB-EP359941EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetolactate synthase isozyme 2 small subunit (ilvM) CSB-YP359942EGX
CSB-EP359942EGX
CSB-BP359942EGX
CSB-MP359942EGX
CSB-EP359942EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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