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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marchantia polymorpha Cytochrome b6 (petB), partial CSB-YP361761MCB1
CSB-EP361761MCB1
CSB-BP361761MCB1
CSB-MP361761MCB1
CSB-EP361761MCB1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP361762MCB1
CSB-EP361762MCB1
CSB-BP361762MCB1
CSB-MP361762MCB1
CSB-EP361762MCB1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP361763NHE
CSB-EP361763NHE
CSB-BP361763NHE
CSB-MP361763NHE
CSB-EP361763NHE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP361764NHE
CSB-EP361764NHE
CSB-BP361764NHE
CSB-MP361764NHE
CSB-EP361764NHE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Protein P (P), partial CSB-YP361766WAT
CSB-EP361766WAT
CSB-BP361766WAT
CSB-MP361766WAT
CSB-EP361766WAT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP361767ENV
CSB-EP361767ENV
CSB-BP361767ENV
CSB-MP361767ENV
CSB-EP361767ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP361768NHE1
CSB-EP361768NHE1
CSB-BP361768NHE1
CSB-MP361768NHE1
CSB-EP361768NHE1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP361769MCB
CSB-EP361769MCB
CSB-BP361769MCB
CSB-MP361769MCB
CSB-EP361769MCB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gibberella zeae Galactose oxidase inhibitor CSB-YP361770GBZ
CSB-EP361770GBZ
CSB-BP361770GBZ
CSB-MP361770GBZ
CSB-EP361770GBZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Pro-opiomelanocortin A (pomc-a) CSB-YP361771XBE
CSB-EP361771XBE
CSB-BP361771XBE
CSB-MP361771XBE
CSB-EP361771XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alligator mississippiensis Pancreatic hormone (ppy) CSB-YP361772AZO
CSB-EP361772AZO
CSB-BP361772AZO
CSB-MP361772AZO
CSB-EP361772AZO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V-II region GM607 CSB-YP361773HU
CSB-EP361773HU
CSB-BP361773HU
CSB-MP361773HU
CSB-EP361773HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V-III region IARC/BL41 CSB-YP361774HU
CSB-EP361774HU
CSB-BP361774HU
CSB-MP361774HU
CSB-EP361774HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V-IV region JI CSB-YP361775HU
CSB-EP361775HU
CSB-BP361775HU
CSB-MP361775HU
CSB-EP361775HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V region EV15 CSB-YP361776HU
CSB-EP361776HU
CSB-BP361776HU
CSB-MP361776HU
CSB-EP361776HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig lambda chain V-VI region SUT CSB-YP361777HU
CSB-EP361777HU
CSB-BP361777HU
CSB-MP361777HU
CSB-EP361777HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig lambda chain V-VI region EB4 CSB-YP361778HU
CSB-EP361778HU
CSB-BP361778HU
CSB-MP361778HU
CSB-EP361778HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse T-cell receptor beta chain V region A20.2.25 CSB-YP361779MO
CSB-EP361779MO
CSB-BP361779MO
CSB-MP361779MO
CSB-EP361779MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse T-cell receptor alpha chain V region CTL-F3 CSB-YP361780MO
CSB-EP361780MO
CSB-BP361780MO
CSB-MP361780MO
CSB-EP361780MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse T-cell receptor gamma chain V region 5/10-13 (Gm16602) CSB-YP361781MO
CSB-EP361781MO
CSB-BP361781MO
CSB-MP361781MO
CSB-EP361781MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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