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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Histidine transport system permease protein hisM (hisM), partial CSB-YP360168SZB1
CSB-EP360168SZB1
CSB-BP360168SZB1
CSB-MP360168SZB1
CSB-EP360168SZB1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein skp (skp) CSB-YP360169EGX
CSB-EP360169EGX
CSB-BP360169EGX
CSB-MP360169EGX
CSB-EP360169EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein skp (skp) CSB-YP360170SZB
CSB-EP360170SZB
CSB-BP360170SZB
CSB-MP360170SZB
CSB-EP360170SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein hnr (hnr) CSB-YP360171EOD
CSB-EP360171EOD
CSB-BP360171EOD
CSB-MP360171EOD
CSB-EP360171EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formate hydrogenlyase regulatory protein hycA (hycA) CSB-YP360172ENV
CSB-EP360172ENV
CSB-BP360172ENV
CSB-MP360172ENV
CSB-EP360172ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formate hydrogenlyase regulatory protein hycA (hycA) CSB-YP360173SZB
CSB-EP360173SZB
CSB-BP360173SZB
CSB-MP360173SZB
CSB-EP360173SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formate hydrogenlyase maturation protein hycH (hycH) CSB-YP360174EOD
CSB-EP360174EOD
CSB-BP360174EOD
CSB-MP360174EOD
CSB-EP360174EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenase 3 maturation protease (hycI) CSB-YP360175EOD
CSB-EP360175EOD
CSB-BP360175EOD
CSB-MP360175EOD
CSB-EP360175EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenase-4 component E (hyfE), partial CSB-YP360176EOD1
CSB-EP360176EOD1
CSB-BP360176EOD1
CSB-MP360176EOD1
CSB-EP360176EOD1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Guanosine-inosine kinase (gsk) CSB-YP360177ENV
CSB-BP360177ENV
CSB-MP360177ENV
CSB-EP360177ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inosine-guanosine kinase (gsk) CSB-YP360178EOD
CSB-EP360178EOD
CSB-BP360178EOD
CSB-MP360178EOD
CSB-EP360178EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-phosphofructokinase (fruK) CSB-YP360179EOD
CSB-EP360179EOD
CSB-BP360179EOD
CSB-MP360179EOD
CSB-EP360179EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Alpha-ketoglutarate permease (kgtP), partial CSB-YP360180ENV
CSB-EP360180ENV
CSB-BP360180ENV
CSB-MP360180ENV
CSB-EP360180ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable phosphoribulokinase (prkB) CSB-YP360181ENV
CSB-EP360181ENV
CSB-BP360181ENV
CSB-MP360181ENV
CSB-EP360181ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli High-affinity branched-chain amino acid transport system permease protein livH (livH), partial CSB-YP360182ENV
CSB-EP360182ENV
CSB-BP360182ENV
CSB-MP360182ENV
CSB-EP360182ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltose/maltodextrin-binding periplasmic protein (malE) CSB-YP360183ENV
CSB-BP360183ENV
CSB-MP360183ENV
CSB-EP360183ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0056 inner membrane protein marC (marC), partial CSB-YP360184ENV1
CSB-EP360184ENV1
CSB-BP360184ENV1
CSB-MP360184ENV1
CSB-EP360184ENV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nucleoside triphosphate pyrophosphohydrolase (mazG) CSB-YP360185ENV
CSB-EP360185ENV
CSB-BP360185ENV
CSB-MP360185ENV
CSB-EP360185ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Modulator of drug activity B (mdaB) CSB-YP360186ENV
CSB-EP360186ENV
CSB-BP360186ENV
CSB-MP360186ENV
CSB-EP360186ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modulator of drug activity B (mdaB) CSB-YP360187EOD
CSB-EP360187EOD
CSB-BP360187EOD
CSB-MP360187EOD
CSB-EP360187EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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