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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nicotiana tabacum 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP361802NHE
CSB-EP361802NHE
CSB-BP361802NHE
CSB-MP361802NHE
CSB-EP361802NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP361803MCB
CSB-EP361803MCB
CSB-BP361803MCB
CSB-MP361803MCB
CSB-EP361803MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP361804NHE
CSB-EP361804NHE
CSB-BP361804NHE
CSB-MP361804NHE
CSB-EP361804NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP361805NHE
CSB-EP361805NHE
CSB-BP361805NHE
CSB-MP361805NHE
CSB-EP361805NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP361806MCB
CSB-EP361806MCB
CSB-BP361806MCB
CSB-MP361806MCB
CSB-EP361806MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha 50S ribosomal protein L23, chloroplastic (rpl23) CSB-YP361807MCB
CSB-EP361807MCB
CSB-BP361807MCB
CSB-MP361807MCB
CSB-EP361807MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP361808MCB
CSB-EP361808MCB
CSB-BP361808MCB
CSB-MP361808MCB
CSB-EP361808MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Troponin T, fast skeletal muscle isoforms (TNNT3) CSB-YP361809DXJ
CSB-EP361809DXJ
CSB-BP361809DXJ
CSB-MP361809DXJ
CSB-EP361809DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Photosystem Q (B) protein, partial CSB-YP361810MCB1
CSB-EP361810MCB1
CSB-BP361810MCB1
CSB-MP361810MCB1
CSB-EP361810MCB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP361811NHE
CSB-EP361811NHE
CSB-BP361811NHE
CSB-MP361811NHE
CSB-EP361811NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP361812NHE
CSB-EP361812NHE
CSB-BP361812NHE
CSB-MP361812NHE
CSB-EP361812NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP361813NHE1
CSB-EP361813NHE1
CSB-BP361813NHE1
CSB-MP361813NHE1
CSB-EP361813NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP361814NHE1
CSB-EP361814NHE1
CSB-BP361814NHE1
CSB-MP361814NHE1
CSB-EP361814NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem II reaction center protein H (psbH), partial CSB-YP361815NHE1
CSB-EP361815NHE1
CSB-BP361815NHE1
CSB-MP361815NHE1
CSB-EP361815NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP361816HOR
CSB-EP361816HOR
CSB-BP361816HOR
CSB-MP361816HOR
CSB-EP361816HOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2), partial CSB-YP361817HOR
CSB-EP361817HOR
CSB-BP361817HOR
CSB-MP361817HOR
CSB-EP361817HOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP361818HNF
CSB-EP361818HNF
CSB-BP361818HNF
CSB-MP361818HNF
CSB-EP361818HNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP361819HNF
CSB-EP361819HNF
CSB-BP361819HNF
CSB-MP361819HNF
CSB-EP361819HNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein E4 (E4) CSB-YP361820HOR
CSB-EP361820HOR
CSB-BP361820HOR
CSB-MP361820HOR
CSB-EP361820HOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human papillomavirus 33 Protein E6 (E6) CSB-YP361821HNF
CSB-EP361821HNF
CSB-BP361821HNF
CSB-MP361821HNF
CSB-EP361821HNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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