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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Mouse Pancreatic alpha-amylase (Amy2) CSB-YP360434MO
CSB-EP360434MO
CSB-BP360434MO
CSB-MP360434MO
CSB-EP360434MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Endolysin (E) CSB-YP360435EDZ
CSB-BP360435EDZ
CSB-MP360435EDZ
CSB-EP360435EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage lambda Endopeptidase (Rz), partial CSB-YP360436ECW
CSB-EP360436ECW
CSB-BP360436ECW
CSB-MP360436ECW
CSB-EP360436ECW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Urokinase-type plasminogen activator (PLAU) CSB-YP360437HU
CSB-BP360437HU
CSB-EP360437HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Glandular kallikrein CSB-YP360438PI
CSB-EP360438PI
CSB-BP360438PI
CSB-MP360438PI
CSB-EP360438PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Kallikrein 1-related peptidase-like b4 (Klk1b4) CSB-YP360439MO
CSB-EP360439MO
CSB-BP360439MO
CSB-MP360439MO
CSB-EP360439MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Chymotrypsinogen B CSB-YP360440BO
CSB-EP360440BO
CSB-BP360440BO
CSB-MP360440BO
CSB-EP360440BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus dysgalactiae subsp. equisimilis Streptokinase C (skc) CSB-YP360442SMK
CSB-EP360442SMK
CSB-BP360442SMK
CSB-MP360442SMK
CSB-EP360442SMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Pepsin A (PGA) CSB-YP360444BO
CSB-EP360444BO
CSB-BP360444BO
CSB-MP360444BO
CSB-EP360444BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Chymosin (CYM) CSB-YP360445BO
CSB-EP360445BO
CSB-BP360445BO
CSB-MP360445BO
CSB-EP360445BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucorpepsin CSB-YP360446RDA
CSB-EP360446RDA
CSB-BP360446RDA
CSB-MP360446RDA
CSB-EP360446RDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Beta-lactamase (ampC) CSB-YP360448ENV
CSB-BP360448ENV
CSB-MP360448ENV
CSB-EP360448ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cyanate hydratase (cynS) CSB-YP360449ENV
CSB-EP360449ENV
CSB-BP360449ENV
CSB-MP360449ENV
CSB-EP360449ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum ATP synthase epsilon chain, chloroplastic (atpE) CSB-YP360450NHE
CSB-EP360450NHE
CSB-BP360450NHE
CSB-MP360450NHE
CSB-EP360450NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP360451ZAX1
CSB-EP360451ZAX1
CSB-BP360451ZAX1
CSB-MP360451ZAX1
CSB-EP360451ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus sp. Histidine decarboxylase proenzyme (hdcA) CSB-YP360452LBD
CSB-EP360452LBD
CSB-BP360452LBD
CSB-MP360452LBD
CSB-EP360452LBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Ribulose bisphosphate carboxylase small chain 1, chloroplastic (RBCS-1) CSB-YP360453GGV
CSB-EP360453GGV
CSB-BP360453GGV
CSB-MP360453GGV
CSB-EP360453GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Ribulose bisphosphate carboxylase small chain (RBCS) CSB-YP360454FKI
CSB-EP360454FKI
CSB-BP360454FKI
CSB-MP360454FKI
CSB-EP360454FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna gibba Ribulose bisphosphate carboxylase small chain SSU1, chloroplastic (SSU1) CSB-YP360455LDU
CSB-EP360455LDU
CSB-BP360455LDU
CSB-MP360455LDU
CSB-EP360455LDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP360456NHE
CSB-EP360456NHE
CSB-BP360456NHE
CSB-MP360456NHE
CSB-EP360456NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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