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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechocystis sp. DNA-binding protein HU (hup) CSB-YP360846SSN
CSB-EP360846SSN
CSB-BP360846SSN
CSB-MP360846SSN
CSB-EP360846SSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein HRL53 CSB-YP360847RKN
CSB-EP360847RKN
CSB-BP360847RKN
CSB-MP360847RKN
CSB-EP360847RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S5 (rpsE) CSB-YP360848GFM
CSB-EP360848GFM
CSB-BP360848GFM
CSB-MP360848GFM
CSB-EP360848GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S7 (rpsG) CSB-YP360849ENV
CSB-BP360849ENV
CSB-MP360849ENV
CSB-EP360849ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 60S ribosomal protein L4-B (rpl4-b) CSB-YP360850XBE
CSB-EP360850XBE
CSB-BP360850XBE
CSB-MP360850XBE
CSB-EP360850XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L7/L12 (rplL) CSB-YP360851BRJ
CSB-EP360851BRJ
CSB-BP360851BRJ
CSB-MP360851BRJ
CSB-EP360851BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L7/L12 (rplL) CSB-YP360852RLE
CSB-EP360852RLE
CSB-BP360852RLE
CSB-MP360852RLE
CSB-EP360852RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S acidic ribosomal protein P2-beta (RPP2B) CSB-YP360853SVG
CSB-EP360853SVG
CSB-BP360853SVG
CSB-MP360853SVG
CSB-EP360853SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S17-A (RPS17A) CSB-YP360854SVG
CSB-EP360854SVG
CSB-BP360854SVG
CSB-MP360854SVG
CSB-EP360854SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L9 (rplI) CSB-YP360855GFM
CSB-EP360855GFM
CSB-BP360855GFM
CSB-MP360855GFM
CSB-EP360855GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Keratin, high-sulfur matrix protein, IIIA3 CSB-YP360856SH
CSB-EP360856SH
CSB-BP360856SH
CSB-MP360856SH
CSB-EP360856SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Keratin, high-sulfur matrix protein, IIIA3A CSB-YP360857SH
CSB-EP360857SH
CSB-BP360857SH
CSB-MP360857SH
CSB-EP360857SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Feather keratin 1 CSB-YP360858CH
CSB-EP360858CH
CSB-BP360858CH
CSB-MP360858CH
CSB-EP360858CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Heat shock protein 26 (Hsp26) CSB-YP360859DLU
CSB-EP360859DLU
CSB-BP360859DLU
CSB-MP360859DLU
CSB-EP360859DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max 17.3 kDa class I heat shock protein (HSP17.3-B) CSB-YP360860GGV
CSB-EP360860GGV
CSB-BP360860GGV
CSB-MP360860GGV
CSB-EP360860GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Keratin, type I microfibrillar 48 kDa, component 8C-1 CSB-YP360861SH
CSB-EP360861SH
CSB-BP360861SH
CSB-MP360861SH
CSB-EP360861SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lytechinus pictus Tubulin alpha chain CSB-YP360862LRW
CSB-EP360862LRW
CSB-BP360862LRW
CSB-MP360862LRW
CSB-EP360862LRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Myosin heavy chain, skeletal muscle, partial CSB-YP360863RB
CSB-EP360863RB
CSB-BP360863RB
CSB-MP360863RB
CSB-EP360863RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Actin, larval muscle (Act79B) CSB-YP360864DLU
CSB-EP360864DLU
CSB-BP360864DLU
CSB-MP360864DLU
CSB-EP360864DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii Actin-1 CSB-YP360865AAJ
CSB-EP360865AAJ
CSB-BP360865AAJ
CSB-MP360865AAJ
CSB-EP360865AAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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