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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Variant surface glycoprotein 7 CSB-YP360908TQX
CSB-EP360908TQX
CSB-BP360908TQX
CSB-MP360908TQX
CSB-EP360908TQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized kinetoplast minicircle 51 polypeptide CSB-YP360909TQV
CSB-EP360909TQV
CSB-BP360909TQV
CSB-MP360909TQV
CSB-EP360909TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylmalonyl-CoA carboxyltransferase 1.3S subunit CSB-YP360910PSH
CSB-EP360910PSH
CSB-BP360910PSH
CSB-MP360910PSH
CSB-EP360910PSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Histidine transport ATP-binding protein HisP (hisP) CSB-YP360911SXB
CSB-EP360911SXB
CSB-BP360911SXB
CSB-MP360911SXB
CSB-EP360911SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltose transport system permease protein malF (malF), partial CSB-YP360912ENV
CSB-EP360912ENV
CSB-BP360912ENV
CSB-MP360912ENV
CSB-EP360912ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Melibiose carrier protein (melB), partial CSB-YP360913ENV
CSB-EP360913ENV
CSB-BP360913ENV
CSB-MP360913ENV
CSB-EP360913ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein tonB (tonB), partial CSB-YP360914ENV
CSB-EP360914ENV
CSB-BP360914ENV
CSB-MP360914ENV
CSB-EP360914ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Outer membrane pore protein E (phoE) CSB-YP360915ENV
CSB-EP360915ENV
CSB-BP360915ENV
CSB-MP360915ENV
CSB-EP360915ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane lipoprotein (lpp) CSB-YP360916SYN
CSB-EP360916SYN
CSB-BP360916SYN
CSB-MP360916SYN
CSB-EP360916SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Methyl-accepting chemotaxis protein II (tar), partial CSB-YP360917SXB
CSB-EP360917SXB
CSB-BP360917SXB
CSB-MP360917SXB
CSB-EP360917SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Tuberculin-active protein CSB-YP360918MVZ
CSB-EP360918MVZ
CSB-BP360918MVZ
CSB-MP360918MVZ
CSB-EP360918MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Light-harvesting protein B-870 alpha chain (pufA), partial CSB-YP360919RLC
CSB-EP360919RLC
CSB-BP360919RLC
CSB-MP360919RLC
CSB-EP360919RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Photosystem Q (B) protein, partial CSB-YP360920GGV1
CSB-EP360920GGV1
CSB-BP360920GGV1
CSB-MP360920GGV1
CSB-EP360920GGV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein gamma-type (sasP-B) CSB-YP360921BQV
CSB-EP360921BQV
CSB-BP360921BQV
CSB-MP360921BQV
CSB-EP360921BQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-H factor (sigH) CSB-YP360922BQT
CSB-EP360922BQT
CSB-BP360922BQT
CSB-MP360922BQT
CSB-EP360922BQT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Development-specific protein S homolog (ops) CSB-YP360923MXA
CSB-EP360923MXA
CSB-BP360923MXA
CSB-MP360923MXA
CSB-EP360923MXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Flagellin fljJ (fljJ) CSB-YP360924DQO
CSB-EP360924DQO
CSB-BP360924DQO
CSB-MP360924DQO
CSB-EP360924DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Immunoglobulin G-binding protein A (spa) CSB-YP360925FLF
CSB-EP360925FLF
CSB-BP360925FLF
CSB-MP360925FLF
CSB-EP360925FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Colicin-E1 (cea), partial CSB-YP360926ENL1
CSB-BP360926ENL1
CSB-MP360926ENL1
CSB-EP360926ENL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracycline resistance protein, class C (tetA), partial CSB-YP360927ENL
CSB-EP360927ENL
CSB-BP360927ENL
CSB-MP360927ENL
CSB-EP360927ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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