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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella typhimurium Nitrogen regulation protein NR (II) CSB-YP363239SXB
CSB-EP363239SXB
CSB-BP363239SXB
CSB-MP363239SXB
CSB-EP363239SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium RNA polymerase sigma factor rpoD (rpoD), partial CSB-YP363240SXB
CSB-EP363240SXB
CSB-BP363240SXB
CSB-MP363240SXB
CSB-EP363240SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma factor rpoS (rpoS) CSB-YP363241SWW
CSB-EP363241SWW
CSB-BP363241SWW
CSB-MP363241SWW
CSB-EP363241SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma factor rpoS (rpoS) CSB-YP363242SWM
CSB-EP363242SWM
CSB-BP363242SWM
CSB-MP363242SWM
CSB-EP363242SWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma factor for flagellar operon (fliA) CSB-YP363243SWW
CSB-EP363243SWW
CSB-BP363243SWW
CSB-MP363243SWW
CSB-EP363243SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-E factor (rpoE) CSB-YP363244SWW
CSB-EP363244SWW
CSB-BP363244SWW
CSB-MP363244SWW
CSB-EP363244SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD-dependent protein deacylase (cobB) CSB-YP363245SWW
CSB-EP363245SWW
CSB-BP363245SWW
CSB-MP363245SWW
CSB-EP363245SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Citrate-sodium symporter (citP), partial CSB-YP363246SXB
CSB-EP363246SXB
CSB-BP363246SXB
CSB-MP363246SXB
CSB-EP363246SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrate-sodium symporter (citP), partial CSB-YP363247SBO
CSB-EP363247SBO
CSB-BP363247SBO
CSB-MP363247SBO
CSB-EP363247SBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP363248SWW
CSB-EP363248SWW
CSB-BP363248SWW
CSB-MP363248SWW
CSB-EP363248SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrate-proton symporter (citA), partial CSB-YP363249SWW
CSB-EP363249SWW
CSB-BP363249SWW
CSB-MP363249SWW
CSB-EP363249SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative sialic acid transporter (nanT), partial CSB-YP363250SWW
CSB-EP363250SWW
CSB-BP363250SWW
CSB-MP363250SWW
CSB-EP363250SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yciH (yciH) CSB-YP363251SWW
CSB-EP363251SWW
CSB-BP363251SWW
CSB-MP363251SWW
CSB-EP363251SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalt-precorrin-4 C (11)-methyltransferase CSB-YP363252SWW
CSB-EP363252SWW
CSB-BP363252SWW
CSB-MP363252SWW
CSB-EP363252SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Sec-independent protein translocase protein TatA (tatA), partial CSB-YP363253SXB
CSB-EP363253SXB
CSB-BP363253SXB
CSB-MP363253SXB
CSB-EP363253SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable Sec-independent protein translocase protein TatE (tatE), partial CSB-YP363254SXB
CSB-EP363254SXB
CSB-BP363254SXB
CSB-MP363254SXB
CSB-EP363254SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Thiol:disulfide interchange protein DsbA (dsbA) CSB-YP363255SXB
CSB-EP363255SXB
CSB-BP363255SXB
CSB-MP363255SXB
CSB-EP363255SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA gyrase subunit B (gyrB), partial CSB-YP363256SXB
CSB-EP363256SXB
CSB-BP363256SXB
CSB-MP363256SXB
CSB-EP363256SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA topoisomerase 4 subunit B (parE), partial CSB-YP363257SXB
CSB-EP363257SXB
CSB-BP363257SXB
CSB-MP363257SXB
CSB-EP363257SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Histidine transport system permease protein hisM (hisM), partial CSB-YP363258SXB1
CSB-EP363258SXB1
CSB-BP363258SXB1
CSB-MP363258SXB1
CSB-EP363258SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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