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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Transcriptional regulator mxiE (mxiE) CSB-YP363301SGH
CSB-EP363301SGH
CSB-BP363301SGH
CSB-MP363301SGH
CSB-EP363301SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional activator rhaS (rhaS) CSB-YP363302SWW
CSB-EP363302SWW
CSB-BP363302SWW
CSB-MP363302SWW
CSB-EP363302SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Virulence regulon transcriptional activator virF (virF) CSB-YP363303SZA
CSB-EP363303SZA
CSB-BP363303SZA
CSB-MP363303SZA
CSB-EP363303SZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Multiple antibiotic resistance protein marR (marR) CSB-YP363304SXB
CSB-EP363304SXB
CSB-BP363304SXB
CSB-MP363304SXB
CSB-EP363304SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Catabolite gene activator (crp) CSB-YP363305SXB
CSB-EP363305SXB
CSB-BP363305SXB
CSB-MP363305SXB
CSB-EP363305SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Fumarate and nitrate reduction regulatory protein (fnr) CSB-YP363306SXB
CSB-EP363306SXB
CSB-BP363306SXB
CSB-MP363306SXB
CSB-EP363306SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ribosomal protein S6 modification protein (rimK) CSB-YP363307SXB
CSB-EP363307SXB
CSB-BP363307SXB
CSB-MP363307SXB
CSB-EP363307SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Chaperone protein ipgC (ipgC) CSB-YP363308SZB
CSB-EP363308SZB
CSB-BP363308SZB
CSB-MP363308SZB
CSB-EP363308SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc transport system ATP-binding protein AdcC (adcC) CSB-YP363309FMW
CSB-EP363309FMW
CSB-BP363309FMW
CSB-MP363309FMW
CSB-EP363309FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligopeptide transport ATP-binding protein AmiE (amiE) CSB-YP363310FMW
CSB-EP363310FMW
CSB-BP363310FMW
CSB-MP363310FMW
CSB-EP363310FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Beta- (1-->2)glucan export ATP-binding/permease protein NdvA, partial CSB-YP363311AYS1
CSB-EP363311AYS1
CSB-BP363311AYS1
CSB-MP363311AYS1
CSB-EP363311AYS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Octopine permease ATP-binding protein P (occP) CSB-YP363312RKV
CSB-EP363312RKV
CSB-BP363312RKV
CSB-MP363312RKV
CSB-EP363312RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Oligopeptide transport ATP-binding protein OppF (oppF) CSB-YP363313LNG
CSB-EP363313LNG
CSB-BP363313LNG
CSB-MP363313LNG
CSB-EP363313LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligopeptide transport ATP-binding protein OppF (oppF) CSB-YP363314SMT
CSB-EP363314SMT
CSB-BP363314SMT
CSB-MP363314SMT
CSB-EP363314SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Phosphate import ATP-binding protein PstB 3 (pstB3) CSB-YP363315SMP
CSB-EP363315SMP
CSB-BP363315SMP
CSB-MP363315SMP
CSB-EP363315SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl carrier protein AcpXL (acpXL) CSB-YP363316RKR
CSB-EP363316RKR
CSB-BP363316RKR
CSB-MP363316RKR
CSB-EP363316RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP363317SMP
CSB-EP363317SMP
CSB-BP363317SMP
CSB-MP363317SMP
CSB-EP363317SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Alanine racemase (alr) CSB-YP363318SMP
CSB-EP363318SMP
CSB-BP363318SMP
CSB-MP363318SMP
CSB-EP363318SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Indoleacetamide hydrolase (tms2) CSB-YP363319AFA
CSB-EP363319AFA
CSB-BP363319AFA
CSB-MP363319AFA
CSB-EP363319AFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease (exoA) CSB-YP363320FMW
CSB-EP363320FMW
CSB-BP363320FMW
CSB-MP363320FMW
CSB-EP363320FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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