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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) CSB-YP363669MVZ
CSB-EP363669MVZ
CSB-BP363669MVZ
CSB-MP363669MVZ
CSB-EP363669MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Group 1 truncated hemoglobin glbN (glbN) CSB-YP363670MVH
CSB-EP363670MVH
CSB-BP363670MVH
CSB-MP363670MVH
CSB-EP363670MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Group 2 truncated hemoglobin glbO (glbO) CSB-YP363671MVZ
CSB-EP363671MVZ
CSB-BP363671MVZ
CSB-MP363671MVZ
CSB-EP363671MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized glycosyltransferase Rv0539/MT0564 (Rv0539, MT0564) CSB-YP363672MVZ
CSB-EP363672MVZ
CSB-BP363672MVZ
CSB-MP363672MVZ
CSB-EP363672MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PGL/p-HBAD biosynthesis glycosyltransferase Rv2957/MT3031 (Rv2957, MT3031) CSB-YP363673MVZ
CSB-EP363673MVZ
CSB-BP363673MVZ
CSB-MP363673MVZ
CSB-EP363673MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent DNA helicase pcrA (pcrA), partial CSB-YP363674MVZ
CSB-EP363674MVZ
CSB-BP363674MVZ
CSB-MP363674MVZ
CSB-EP363674MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein Mb2227c (Mb2227c) CSB-YP363676MVH
CSB-EP363676MVH
CSB-BP363676MVH
CSB-MP363676MVH
CSB-EP363676MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP363677MVZ
CSB-EP363677MVZ
CSB-BP363677MVZ
CSB-MP363677MVZ
CSB-EP363677MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP363678MVZ
CSB-EP363678MVZ
CSB-BP363678MVZ
CSB-MP363678MVZ
CSB-EP363678MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HIT-like protein Rv0759c/MT0784 (Rv0759c, MT0784) CSB-YP363679MVZ
CSB-EP363679MVZ
CSB-BP363679MVZ
CSB-MP363679MVZ
CSB-EP363679MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP363680MVZ
CSB-EP363680MVZ
CSB-BP363680MVZ
CSB-MP363680MVZ
CSB-EP363680MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized MFS-type transporter Rv0037c/MT0042 (Rv0037c, MT0042), partial CSB-YP363681MVZ
CSB-EP363681MVZ
CSB-BP363681MVZ
CSB-MP363681MVZ
CSB-EP363681MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0088/MT0096 (Rv0088, MT0096) CSB-YP363682MVZ
CSB-EP363682MVZ
CSB-BP363682MVZ
CSB-MP363682MVZ
CSB-EP363682MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative secreted protein Rv0236.1/MT0250 (Rv0236.1, MT0250) CSB-YP363683MVZ
CSB-EP363683MVZ
CSB-BP363683MVZ
CSB-MP363683MVZ
CSB-EP363683MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0441c/MT0457 (Rv0441c, MT0457) CSB-YP363684MVZ
CSB-EP363684MVZ
CSB-BP363684MVZ
CSB-MP363684MVZ
CSB-EP363684MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0500B/MT0521.1 (Rv0500B, MT0521.1) CSB-YP363685MVZ
CSB-EP363685MVZ
CSB-BP363685MVZ
CSB-MP363685MVZ
CSB-EP363685MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0501/MT0522 (Rv0501, MT0522) CSB-YP363686MVZ
CSB-EP363686MVZ
CSB-BP363686MVZ
CSB-MP363686MVZ
CSB-EP363686MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0874c/MT0897 (Rv0874c, MT0897), partial CSB-YP363687MVZ1
CSB-EP363687MVZ1
CSB-BP363687MVZ1
CSB-MP363687MVZ1
CSB-EP363687MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv0885/MT0908 (Rv0885, MT0908), partial CSB-YP363688MVZ1
CSB-EP363688MVZ1
CSB-BP363688MVZ1
CSB-MP363688MVZ1
CSB-EP363688MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb0984 (Mb0984), partial CSB-YP363689MVH
CSB-EP363689MVH
CSB-BP363689MVH
CSB-MP363689MVH
CSB-EP363689MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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