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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acetate kinase (ackA) CSB-YP363835EGX
CSB-EP363835EGX
CSB-BP363835EGX
CSB-MP363835EGX
CSB-EP363835EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP363836ENV
CSB-EP363836ENV
CSB-BP363836ENV
CSB-MP363836ENV
CSB-EP363836ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine racemase, biosynthetic (alr) CSB-YP363837SZB
CSB-EP363837SZB
CSB-BP363837SZB
CSB-MP363837SZB
CSB-EP363837SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Endonuclease 4 (nfo) CSB-YP363838ENV
CSB-EP363838ENV
CSB-BP363838ENV
CSB-MP363838ENV
CSB-EP363838ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Amino-acid acetyltransferase (argA) CSB-YP363839ENV
CSB-EP363839ENV
CSB-BP363839ENV
CSB-MP363839ENV
CSB-EP363839ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amino-acid acetyltransferase (argA) CSB-YP363840EOD
CSB-EP363840EOD
CSB-BP363840EOD
CSB-MP363840EOD
CSB-EP363840EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylglutamate kinase (argB) CSB-YP363841EOD
CSB-EP363841EOD
CSB-BP363841EOD
CSB-MP363841EOD
CSB-EP363841EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine repressor (argR) CSB-YP363842EOD
CSB-EP363842EOD
CSB-BP363842EOD
CSB-MP363842EOD
CSB-EP363842EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP363843ENV
CSB-BP363843ENV
CSB-MP363843ENV
CSB-EP363843ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Quinate/shikimate dehydrogenase (ydiB) CSB-YP363844ENV
CSB-EP363844ENV
CSB-BP363844ENV
CSB-MP363844ENV
CSB-EP363844ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Shikimate kinase 1 (aroK) CSB-YP363845ENV
CSB-EP363845ENV
CSB-BP363845ENV
CSB-MP363845ENV
CSB-EP363845ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate kinase 1 (aroK) CSB-YP363846EOD
CSB-EP363846EOD
CSB-BP363846EOD
CSB-MP363846EOD
CSB-EP363846EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Shikimate kinase 2 (aroL) CSB-YP363847ENV
CSB-EP363847ENV
CSB-BP363847ENV
CSB-MP363847ENV
CSB-EP363847ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate kinase 2 (aroL) CSB-YP363848EOD
CSB-EP363848EOD
CSB-BP363848EOD
CSB-MP363848EOD
CSB-EP363848EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP363849EGX
CSB-EP363849EGX
CSB-BP363849EGX
CSB-MP363849EGX
CSB-EP363849EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent dethiobiotin synthetase BioD 2 (bioD2) CSB-YP363850ENV
CSB-EP363850ENV
CSB-BP363850ENV
CSB-MP363850ENV
CSB-EP363850ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Carbamoyl-phosphate synthase small chain (carA) CSB-YP363851ENV
CSB-EP363851ENV
CSB-BP363851ENV
CSB-MP363851ENV
CSB-EP363851ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerol kinase (glpK) CSB-YP363852ENV
CSB-EP363852ENV
CSB-BP363852ENV
CSB-MP363852ENV
CSB-EP363852ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 60 kDa chaperonin (groL) CSB-YP363853ENV
CSB-EP363853ENV
CSB-BP363853ENV
CSB-MP363853ENV
CSB-EP363853ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL) CSB-YP363854EOD
CSB-EP363854EOD
CSB-BP363854EOD
CSB-MP363854EOD
CSB-EP363854EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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