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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Uridine kinase (udk) CSB-YP364158EOD
CSB-EP364158EOD
CSB-BP364158EOD
CSB-MP364158EOD
CSB-EP364158EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UvrABC system protein B (uvrB) CSB-YP364159ENV
CSB-EP364159ENV
CSB-BP364159ENV
CSB-MP364159ENV
CSB-EP364159ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UvrABC system protein C (uvrC) CSB-YP364160ENV
CSB-EP364160ENV
CSB-BP364160ENV
CSB-MP364160ENV
CSB-EP364160ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uronate isomerase (uxaC) CSB-YP364161ENV
CSB-EP364161ENV
CSB-BP364161ENV
CSB-MP364161ENV
CSB-EP364161ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uronate isomerase (uxaC) CSB-YP364162SZB
CSB-EP364162SZB
CSB-BP364162SZB
CSB-MP364162SZB
CSB-EP364162SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavoprotein WrbA (wrbA) CSB-YP364163EGX
CSB-EP364163EGX
CSB-BP364163EGX
CSB-MP364163EGX
CSB-EP364163EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavoprotein WrbA (wrbA) CSB-YP364164SZB
CSB-EP364164SZB
CSB-BP364164SZB
CSB-MP364164SZB
CSB-EP364164SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP364165EGX
CSB-EP364165EGX
CSB-BP364165EGX
CSB-MP364165EGX
CSB-EP364165EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP364166SZB
CSB-EP364166SZB
CSB-BP364166SZB
CSB-MP364166SZB
CSB-EP364166SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc transporter ZupT (zupT), partial CSB-YP364167EGX
CSB-EP364167EGX
CSB-BP364167EGX
CSB-MP364167EGX
CSB-EP364167EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Der GTPase-activating protein YihI (yihI) CSB-YP364168ENV
CSB-EP364168ENV
CSB-BP364168ENV
CSB-MP364168ENV
CSB-EP364168ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA gyrase inhibitor YacG (yacG) CSB-YP364169ENV
CSB-EP364169ENV
CSB-BP364169ENV
CSB-MP364169ENV
CSB-EP364169ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase inhibitor YacG (yacG) CSB-YP364170SZB
CSB-EP364170SZB
CSB-BP364170SZB
CSB-MP364170SZB
CSB-EP364170SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative Holliday junction resolvase (yqgF) CSB-YP364171EGX
CSB-EP364171EGX
CSB-BP364171EGX
CSB-MP364171EGX
CSB-EP364171EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0246 protein yaaA (yaaA) CSB-YP364172SZB
CSB-EP364172SZB
CSB-BP364172SZB
CSB-MP364172SZB
CSB-EP364172SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (7)-)-methyltransferase CSB-YP364173EOD
CSB-EP364173EOD
CSB-BP364173EOD
CSB-MP364173EOD
CSB-EP364173EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP364174ENV
CSB-EP364174ENV
CSB-BP364174ENV
CSB-MP364174ENV
CSB-EP364174ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP364175EOD
CSB-EP364175EOD
CSB-BP364175EOD
CSB-MP364175EOD
CSB-EP364175EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Primosomal protein 1 (dnaT) CSB-YP364176ENV
CSB-EP364176ENV
CSB-BP364176ENV
CSB-MP364176ENV
CSB-EP364176ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0250 protein ybeD (ybeD) CSB-YP364177ENV
CSB-EP364177ENV
CSB-BP364177ENV
CSB-MP364177ENV
CSB-EP364177ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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