Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Formate hydrogenlyase maturation protein hycH (hycH) CSB-YP365206ENV
CSB-EP365206ENV
CSB-BP365206ENV
CSB-MP365206ENV
CSB-EP365206ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase 3 maturation protease (hycI) CSB-YP365207ENV
CSB-EP365207ENV
CSB-BP365207ENV
CSB-MP365207ENV
CSB-EP365207ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase-4 component E (hyfE), partial CSB-YP365208ENV1
CSB-EP365208ENV1
CSB-BP365208ENV1
CSB-MP365208ENV1
CSB-EP365208ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenase-4 component E (hyfE), partial CSB-YP365209SZB1
CSB-EP365209SZB1
CSB-BP365209SZB1
CSB-MP365209SZB1
CSB-EP365209SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inosine-guanosine kinase (gsk) CSB-YP365210EGX
CSB-EP365210EGX
CSB-BP365210EGX
CSB-MP365210EGX
CSB-EP365210EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 1-phosphofructokinase (fruK) CSB-YP365211ENV
CSB-EP365211ENV
CSB-BP365211ENV
CSB-MP365211ENV
CSB-EP365211ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-phosphofructokinase (fruK) CSB-YP365212EGX
CSB-EP365212EGX
CSB-BP365212EGX
CSB-MP365212EGX
CSB-EP365212EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-phosphofructokinase (fruK) CSB-YP365213SZB
CSB-EP365213SZB
CSB-BP365213SZB
CSB-MP365213SZB
CSB-EP365213SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-ketoglutarate permease (kgtP), partial CSB-YP365214SZB
CSB-EP365214SZB
CSB-BP365214SZB
CSB-MP365214SZB
CSB-EP365214SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Probable phosphoribulokinase (prkB) CSB-YP365215SZB
CSB-EP365215SZB
CSB-BP365215SZB
CSB-MP365215SZB
CSB-EP365215SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant High-affinity branched-chain amino acid transport system permease protein livH (livH), partial CSB-YP365216EOD
CSB-EP365216EOD
CSB-BP365216EOD
CSB-MP365216EOD
CSB-EP365216EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Maltose/maltodextrin-binding periplasmic protein (malE) CSB-YP365217EOD
CSB-EP365217EOD
CSB-BP365217EOD
CSB-MP365217EOD
CSB-EP365217EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0056 inner membrane protein marC (marC), partial CSB-YP365218SZB1
CSB-EP365218SZB1
CSB-BP365218SZB1
CSB-MP365218SZB1
CSB-EP365218SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Nucleoside triphosphate pyrophosphohydrolase (mazG) CSB-YP365219EOD
CSB-EP365219EOD
CSB-BP365219EOD
CSB-MP365219EOD
CSB-EP365219EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modulator of drug activity B (mdaB) CSB-YP365220EGX
CSB-EP365220EGX
CSB-BP365220EGX
CSB-MP365220EGX
CSB-EP365220EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug transporter MdfA (mdfA), partial CSB-YP365221ENV
CSB-EP365221ENV
CSB-BP365221ENV
CSB-MP365221ENV
CSB-EP365221ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug transporter MdfA (mdfA), partial CSB-YP365222EOD
CSB-EP365222EOD
CSB-BP365222EOD
CSB-MP365222EOD
CSB-EP365222EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5,10-methylenetetrahydrofolate reductase (metF) CSB-YP365223SZB
CSB-EP365223SZB
CSB-BP365223SZB
CSB-MP365223SZB
CSB-EP365223SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Septum site-determining protein MinD (minD) CSB-YP365224EGX
CSB-EP365224EGX
CSB-BP365224EGX
CSB-MP365224EGX
CSB-EP365224EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Septum site-determining protein MinD (minD) CSB-YP365225SZB
CSB-EP365225SZB
CSB-BP365225SZB
CSB-MP365225SZB
CSB-EP365225SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>