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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arthrobacter sp. 30S ribosomal protein S15 (rpsO) CSB-YP367026AVA
CSB-EP367026AVA
CSB-BP367026AVA
CSB-MP367026AVA
CSB-EP367026AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP367027AVA
CSB-EP367027AVA
CSB-BP367027AVA
CSB-MP367027AVA
CSB-EP367027AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L35 (rpmI) CSB-YP367028AVA
CSB-EP367028AVA
CSB-BP367028AVA
CSB-MP367028AVA
CSB-EP367028AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP367029AVA
CSB-EP367029AVA
CSB-BP367029AVA
CSB-MP367029AVA
CSB-EP367029AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Transcriptional repressor NrdR (nrdR) CSB-YP367030AVA
CSB-EP367030AVA
CSB-BP367030AVA
CSB-MP367030AVA
CSB-EP367030AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. ATP phosphoribosyltransferase (hisG) CSB-YP367031AVA
CSB-EP367031AVA
CSB-BP367031AVA
CSB-MP367031AVA
CSB-EP367031AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UvrABC system protein B (uvrB), partial CSB-YP367032AVA
CSB-EP367032AVA
CSB-BP367032AVA
CSB-MP367032AVA
CSB-EP367032AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Undecaprenyl-diphosphatase (uppP), partial CSB-YP367033AVA1
CSB-EP367033AVA1
CSB-BP367033AVA1
CSB-MP367033AVA1
CSB-EP367033AVA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Proteasome subunit beta (prcB) CSB-YP367034AVA
CSB-EP367034AVA
CSB-BP367034AVA
CSB-MP367034AVA
CSB-EP367034AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Pup--protein ligase (pafA) CSB-YP367035AVA
CSB-EP367035AVA
CSB-BP367035AVA
CSB-MP367035AVA
CSB-EP367035AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Heat-inducible transcription repressor HrcA (hrcA) CSB-YP367036AVA
CSB-EP367036AVA
CSB-BP367036AVA
CSB-MP367036AVA
CSB-EP367036AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S20 (rpsT) CSB-YP367037AVA
CSB-EP367037AVA
CSB-BP367037AVA
CSB-MP367037AVA
CSB-EP367037AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. N utilization substance protein B homolog (nusB) CSB-YP367038AVA
CSB-EP367038AVA
CSB-BP367038AVA
CSB-MP367038AVA
CSB-EP367038AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Alanine--tRNA ligase (alaS), partial CSB-YP367039AVA
CSB-EP367039AVA
CSB-BP367039AVA
CSB-MP367039AVA
CSB-EP367039AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP367040AVA
CSB-EP367040AVA
CSB-BP367040AVA
CSB-MP367040AVA
CSB-EP367040AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Threonine--tRNA ligase (thrS), partial CSB-YP367041AVA
CSB-EP367041AVA
CSB-BP367041AVA
CSB-MP367041AVA
CSB-EP367041AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Xylose isomerase (xylA) CSB-YP367042AVA
CSB-EP367042AVA
CSB-BP367042AVA
CSB-MP367042AVA
CSB-EP367042AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Ribonuclease HII (rnhB) CSB-YP367043AVA
CSB-EP367043AVA
CSB-BP367043AVA
CSB-MP367043AVA
CSB-EP367043AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Ribosome maturation factor RimM (rimM) CSB-YP367044AVA
CSB-EP367044AVA
CSB-BP367044AVA
CSB-MP367044AVA
CSB-EP367044AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Phosphopantetheine adenylyltransferase (coaD) CSB-YP367045AVA
CSB-EP367045AVA
CSB-BP367045AVA
CSB-MP367045AVA
CSB-EP367045AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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