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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human T-cell leukemia virus 1 Accessory protein p12I, partial CSB-YP366266HQJ1
CSB-EP366266HQJ1
CSB-BP366266HQJ1
CSB-MP366266HQJ1
CSB-EP366266HQJ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (tax) CSB-YP366267HQH
CSB-EP366267HQH
CSB-BP366267HQH
CSB-MP366267HQH
CSB-EP366267HQH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pythium vexans Elicitin Vex1 CSB-YP366268PXY
CSB-EP366268PXY
CSB-BP366268PXY
CSB-MP366268PXY
CSB-EP366268PXY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus amadis Contryphan-Am CSB-YP366269CNT
CSB-EP366269CNT
CSB-BP366269CNT
CSB-MP366269CNT
CSB-EP366269CNT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus magus Iota-conotoxin-like M11.1 CSB-YP366270COS
CSB-EP366270COS
CSB-BP366270COS
CSB-MP366270COS
CSB-EP366270COS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yibT (yibT) CSB-YP366271EOD
CSB-EP366271EOD
CSB-BP366271EOD
CSB-MP366271EOD
CSB-EP366271EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YBL100W-C (YBL100W-C) CSB-YP366272SVG
CSB-EP366272SVG
CSB-BP366272SVG
CSB-MP366272SVG
CSB-EP366272SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger A20 domain-containing stress-associated protein 18 (SAP18) CSB-YP366273OFG
CSB-EP366273OFG
CSB-BP366273OFG
CSB-MP366273OFG
CSB-EP366273OFG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Androctonus crassicauda Toxin Acra I-3 CSB-YP366274AKA
CSB-EP366274AKA
CSB-BP366274AKA
CSB-MP366274AKA
CSB-EP366274AKA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Androctonus crassicauda Toxin a CSB-YP366275AKA
CSB-EP366275AKA
CSB-BP366275AKA
CSB-MP366275AKA
CSB-EP366275AKA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria gracilenta Caerin-1.18 CSB-YP366276LHM
CSB-EP366276LHM
CSB-BP366276LHM
CSB-MP366276LHM
CSB-EP366276LHM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus virgo Conotoxin ViVB CSB-YP366277DWM
CSB-EP366277DWM
CSB-BP366277DWM
CSB-MP366277DWM
CSB-EP366277DWM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA reductase (hemA) CSB-YP366278CMB
CSB-EP366278CMB
CSB-BP366278CMB
CSB-MP366278CMB
CSB-EP366278CMB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At1g65760 (At1g65760) CSB-YP366279DOA
CSB-EP366279DOA
CSB-BP366279DOA
CSB-MP366279DOA
CSB-EP366279DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At4g39756 (At4g39756) CSB-YP366280DOA
CSB-EP366280DOA
CSB-BP366280DOA
CSB-MP366280DOA
CSB-EP366280DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At1g47800 (At1g47800) CSB-YP366281DOA
CSB-EP366281DOA
CSB-BP366281DOA
CSB-MP366281DOA
CSB-EP366281DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Uncharacterized protein SPD_2303 homolog (orf3) CSB-YP366282FMW
CSB-EP366282FMW
CSB-BP366282FMW
CSB-MP366282FMW
CSB-EP366282FMW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L27-B (RPL27B) CSB-YP366283SVG
CSB-EP366283SVG
CSB-BP366283SVG
CSB-MP366283SVG
CSB-EP366283SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-LR2 Gag-Pol polyprotein (TY1B-LR2), partial CSB-YP366284SVG
CSB-EP366284SVG
CSB-BP366284SVG
CSB-MP366284SVG
CSB-EP366284SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-PR2 Gag-Pol polyprotein (TY1B-PR2), partial CSB-YP366285SVG
CSB-EP366285SVG
CSB-BP366285SVG
CSB-MP366285SVG
CSB-EP366285SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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